PXD021505 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis |
Description | Targeted protein degradation via the ubiquitin proteasome has been established in eukaryotes, however no application has been shown in bacteria so far. In the present work, selective protein degradation is shown using hetero bifunctional drugs, bringing substrates in close proximity to the bacterial ClpC1P1P2 protein degradation complex. We performed a TMT based quantitative proteomics analysis to monitor degradation of human BRDT in Mycobacterium smegmatis. Our data shows selective degradation of BRDT when using bacterial PROTACS, indicating the first successful application of targeted protein degradation in bacteria. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-11_17:24:30.501.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Hoi |
SpeciesList | scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-16 01:22:35 | ID requested | |
⏵ 1 | 2022-08-11 17:24:31 | announced | |
Publication List
Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T, BacPROTACs mediate targeted protein degradation in bacteria. Cell, 185(13):2338-2353.e18(2022) [pubmed] |
Keyword List
submitter keyword: TPD, targeted protein degradation, protease, Mycobacteria |
Contact List
Markus Hartl |
contact affiliation | Max Perutz Labs Vienna, Mass Spectrometry Facility, Austria |
contact email | markus.hartl@univie.ac.at |
lab head | |
David Hoi |
contact affiliation | IMP |
contact email | david.hoi@imp.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021505
- Label: PRIDE project
- Name: A quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis