PXD021493 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrated Redox Proteomic Analysis Highlights New Mechanisms of Sensitivity to Silver Nanoparticles |
Description | Silver nanoparticles (AgNPs) are widely used nanomaterials in both commercial and clinical biomedical applications, but the molecular mechanisms underlying their activity remain elusive. In this work we profiled proteomics and redox proteomics changes induced by AgNPs in two lung cancer cell lines: AgNPs sensitive Calu-1 and AgNPs resistant NCI-H358. We show that AgNPs induce changes in protein abundance and reversible oxidation in a time and cell line dependent manner impacting key cellular processes such as protein translation and modification, lipid metabolism, bioenergetics and mitochondrial dynamics. Supporting confocal microscopy and transmission electron microscopy (TEM) data further emphasize mitochondria as a target of AgNPs toxicity differentially impacting mitochondrial networks and morphology in Calu-1 and NCI-H358 lung cells. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-01 |
AnnouncementXML | Submission_2021-04-01_07:27:43.222.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021493 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jingyun Lee |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-15 09:31:20 | ID requested | |
⏵ 1 | 2021-04-01 07:27:43 | announced | |
Publication List
Holmila R, Wu H, Lee J, Tsang AW, Singh R, Furdui CM, Integrated Redox Proteomic Analysis Highlights New Mechanisms of Sensitivity to Silver Nanoparticles. Mol Cell Proteomics, 20():100073(2021) [pubmed] |
Keyword List
submitter keyword: Silver Nanoparticles, Lung, Proteomics, Reversible Protein Oxidation, Mitochondria |
Contact List
Cristina M. Furdui |
contact affiliation | Section on Molecular Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157 |
contact email | cfurdui@wakehealth.edu |
lab head | |
Jingyun Lee |
contact affiliation | Wake Forest Baptist Health |
contact email | jilee@wakehealth.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD021493 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021493
- Label: PRIDE project
- Name: Integrated Redox Proteomic Analysis Highlights New Mechanisms of Sensitivity to Silver Nanoparticles