PXD021460-1

PXD021460 is an original dataset announced via ProteomeXchange.

Title	The temperature quickly modulates protein expression by Codon Usage in wheat filling grain
Description	The protein content determines the cell state. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are largely unknown. We quantified 47,535 mRNAs and 3,742 proteins in filling grain of wheat under two thermal environments. The impact of mRNA abundance and sequence features which implicated in protein translation and degradation on protein expression was evaluated by regression analysis. Transcription, codon usage and amino acid frequency mainly drive the changes in protein expression under heat stress, and their combined contribution explains 58.2% and 66.4% of protein variation in 30 and 40 °C, respectively. Of which, transcription contributes more to the alteration in protein content under 40 °C (31%) than to 30 °C (6%). Codon usage plays a stable and powerful role in protein expression under heat stress, even surpassing transcription. What’s more, the usage of AAG is a key factor regulating rapid protein expression under heat stress.
HostingRepository	PRIDE
AnnounceDate	2021-03-24
AnnouncementXML	Submission_2021-03-23_19:21:05.015.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bingjin Wu
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-14 02:07:43	ID requested
⏵ 1	2021-03-23 19:21:05	announced

Dataset with its publication pending

submitter keyword: Heat stress

Post-transcriptional regulation

Codon usage

AAG frequency

Wheat

Bingjin Wu
contact affiliation	Northwest A&F University, Yangling, Shaanxi, 712100, China
contact email	wbj2010@163.com
lab head
Bingjin Wu
contact affiliation	Northwest A&F University
contact email	wubingjin2017@nwafu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021460
     1. Label: PRIDE project
     2. Name: The temperature quickly modulates protein expression by Codon Usage in wheat filling grain