PXD021437-1

PXD021437 is an original dataset announced via ProteomeXchange.

Title	Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
Description	The probability of synaptic vesicle (SV) fusion in response to an action potential, termed vesicular release probability (Pr), is a stochastic but tightly regulated process. SNARE proteins are the molecular executors of SV fusion and therefore, key modulators of Pr. The non-canonical SNARE VAMP4 is a cargo for activity-dependent bulk endocytosis (ADBE), a major SV retrieval mode operating during intense neuronal activity. Using optical imaging in primary neuronal cultures and slice electrophysiology from wild-type and VAMP4 knockout mice, we reveal that modulation of VAMP4 copy number on SVs is an important mechanism to regulate Pr. The control of VAMP4 copy number is determined by its clearance from axons via the endolysosomal system after its retrieval by ADBE. This activity-dependent regulatory mechanism enables synapses to 1) adapt their Pr dynamically to changing input and 2) couple regulation of synaptic strength to neuronal proteostasis.
HostingRepository	PRIDE
AnnounceDate	2021-03-11
AnnouncementXML	Submission_2021-03-11_03:57:57.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-11 06:47:30	ID requested
⏵ 1	2021-03-11 03:57:58	announced

Dataset with its publication pending

submitter keyword: Synaptic vesicle, endocytosis, VAMP4, endosome, lysosome, neurotransmitter, release probability, synaptic plasticity

Prof.Matthias Trost
contact affiliation	Professor of Proteomics at the Faculty for Medical Sciences at Newcastle University.
contact email	Matthias.Trost@newcastle.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021437
     1. Label: PRIDE project
     2. Name: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes