PXD021426 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Can cheese mites, maggots and molds enhance bioactivity? Peptidomic investigation of functional peptides in four traditional cheeses |
Description | Aside from their amino acid content, dairy proteins are valuable for their ability to carry encrypted bioactive peptides whose activities are latent until released by digestive enzymes or endogenous enzymes within the food. Peptides can possess a wide variety of functionalities, such as antibacterial, antihypertensive, and antioxidative properties, as demonstrated by in vitro and in vivo studies. This phenomenon raises the question as to what impact various traditional cheese-making processes have on the formation of bioactive peptides in the resulting products. In this study, we have profiled the naturally-occurring peptides in two hard and two soft traditional cheeses and have identified their known bioactive sequences. While past studies have typically identified fewer than 100 peptide sequences in a single cheese, we have used modern instrumentation to identify between 2900 and 4700 sequences per cheese, an increase by a factor of about 50. We demonstrated substantial variations in proteolysis and peptide formation between the interior and rind of each cheese, which we ascribed to the differences in microbial composition between these regions. We identified a total of 111 bioactive sequences among the four cheeses, with the greatest number of sequences, 89, originating from Mimolette. The most common bioactivities identified were antimicrobial and inhibition of the angiotensin-converting enzyme. This work revealed that cheese proteolysis and the resulting peptidomes are more complex than originally thought in terms of the number of peptides released, variation in peptidome across sites within a single cheese, and variation in bioactive peptides among cheese-making techniques. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-23 |
AnnouncementXML | Submission_2020-12-23_01:53:49.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021426 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Randall Robinson |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Ovis aries; NCBI TaxID: 9940; |
ModificationList | phosphorylated residue; monohydroxylated residue; deaminated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-11 00:35:47 | ID requested | |
⏵ 1 | 2020-12-23 01:53:50 | announced | |
2 | 2021-01-04 06:37:52 | announced | 2021-01-04: Updated publication reference for PubMed record(s): 33346308. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cheese, peptides, mass spectrometry, bioactive |
Contact List
Daniela Barile |
contact affiliation | Department of Food Science & Technology University of California, Davis Davis, CA, USA |
contact email | dbarile@ucdavis.edu |
lab head | |
Randall Robinson |
contact affiliation | UC Davis |
contact email | rcrobinson@ucdavis.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021426
- Label: PRIDE project
- Name: Can cheese mites, maggots and molds enhance bioactivity? Peptidomic investigation of functional peptides in four traditional cheeses