PXD021371-1

PXD021371 is an original dataset announced via ProteomeXchange.

Title	DCLK1 small extracellular vesicle proteome
Description	Doublecortin-like kinase 1 (DCLK1) is a putative cancer stem cell marker, a promising diagnostic and prognostic maker for malignant tumors and a proposed driver gene for gastric cancer. Here we show that DCLK1 expression in the gastric cancer cell line MKN1 impacts EV biogenesis both quantitatively and qualitatively in a kinase dependent manner, revealing a hitherto unknown role for this putative oncogenic kinase. Importantly, the proteome of EVs isolated from DCLK1 expressing gastric cancer cells is enriched for proteins involved in tumor-promoting biological processes, raising the prospect of establishing DCLK1 as a novel anti-cancer target.
HostingRepository	PRIDE
AnnounceDate	2021-03-23
AnnouncementXML	Submission_2021-03-22_23:10:57.792.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	david greening
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-09 01:09:50	ID requested
⏵ 1	2021-03-22 23:10:58	announced

Dataset with its publication pending

submitter keyword: DCLK1, proteomics, exosomes, extracellular vesicles, migration

David Greening
contact affiliation	Baker Institute
contact email	david.greening@baker.edu.au
lab head
david greening
contact affiliation	Baker Heart and Diabetes Inst.
contact email	david.greening@baker.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021371
     1. Label: PRIDE project
     2. Name: DCLK1 small extracellular vesicle proteome