PXD021296-1

PXD021296 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization
Description	We describe theoretical concepts and explain practical procedures of an electrospray mass spectrometry method, termed “Intact Transmission Epitope Mapping – Thermodynamic Weak-force Order (ITEM-TWO)”, by which can be determined apparent binding energies and dissociation constants of immune complex dissociation reactions in the gas phase. The protocol was developed using natural protein-ligand complexes (myoglobin and RNAse S) and was tested with two immune complexes (FLAG peptide - antiFLAG antibody and Troponin I epitope peptide - anti-Troponin I antibody). ITEM-TWO is a rapid method which requires very little sample consumption for identification of protein bound ligands and for determination of the gas phase protein-ligand complex binding strength.
HostingRepository	PRIDE
AnnounceDate	2020-10-29
AnnouncementXML	Submission_2020-10-29_04:15:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Equus caballus; NCBI TaxID: 9796; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-04 02:49:06	ID requested
⏵ 1	2020-10-29 04:15:45	announced

Danquah BD, Opuni KFM, Roewer C, Koy C, Glocker MO, Mass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization. Molecules, 25(20):(2020) [pubmed]

submitter keyword: Offline ESI-MS, ITEM-TWO, myoglobin, RNAse S, FLAG-peptide–antiFLAG antibody complex, Troponin I epitope peptide-antiTroponin I antibody complex, protocol

Prof. Dr. Michael O. Glocker
contact affiliation	Director, Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021296
     1. Label: PRIDE project
     2. Name: Mass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization