PXD021285-2

PXD021285 is an original dataset announced via ProteomeXchange.

Title	Molecular profiling of tumor-derived exosomes in plasma of patients with melanoma
Description	By isolation of exosomes from body fluids such as serum or plasma with the use of a gel filtration method developed for this purpose, we showed that the level of exosomes in patients with melanoma is higher and may be associated with progression of the tumor and treatment response. By applying the techniques of mass spectrometry we would like to assess the protein profile of exosomes released by melanoma cells and determine whether these changes correlate with selected clinical parameters. The model tests are exosomes released in vivo from melanoma cells (MMTEX). Proteins of exosomes were subjected to qualitative and quantitative analysis by LC-MS/MS. Proteins were digested by trypsin and were subsequently purified on C18 StageTips which significantly improves the chromatographic separation and allows identification of a higher number of proteins. In addition, after digestion the obtained peptides were fractionated and separated using ion chromatography and reversed-phase chromatography (C18 column), and then analysed by tandem mass spectrometry analysis. To evaluate protein content and verify the presence of exosomes we were used the Western blot technique or flow cytometry.
HostingRepository	PRIDE
AnnounceDate	2021-08-12
AnnouncementXML	Submission_2021-08-12_21:41:39.415.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Monika Pietrowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-03 01:40:23	ID requested
1	2021-08-12 00:23:34	announced
⏵ 2	2021-08-12 21:41:40	announced	2021-08-13: Updated publication reference for PubMed record(s): 33613873. 2021-08-13: Updated publication reference for DOI(s): 10.1002/JEV2.12063.
3	2023-11-14 08:51:28	announced	2023-11-14: Updated project metadata.

10.1002/JEV2.12063;

Pietrowska M, Zebrowska A, Gawin M, Marczak L, Sharma P, Mondal S, Mika J, Pola, ń, ska J, Ferrone S, Kirkwood JM, Widlak P, Whiteside TL, Proteomic profile of melanoma cell-derived small extracellular vesicles in patients' plasma: a potential correlate of melanoma progression. J Extracell Vesicles, 10(4):e12063(2021) [pubmed]

submitter keyword: human, plasma, small extracellular vesicles (sEV), tumor-derived exosomes (TEX), melanoma cell-derived exosomes (MTEX), proteomics, high-resolution mass spectrometry (HRMS)

Piotr Widłak
contact affiliation	Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch
contact email	piotr.widlak@io.gliwice.pl
lab head
Monika Pietrowska
contact affiliation	Maria Sklodowska-Curie National Research Institute of Oncology
contact email	monika.pietrowska@io.gliwice.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD021285

PRIDE project URI

• PRIDE
  1. PXD021285
     1. Label: PRIDE project
     2. Name: Molecular profiling of tumor-derived exosomes in plasma of patients with melanoma