PXD021281-1

PXD021281 is an original dataset announced via ProteomeXchange.

Title	Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic and 1 degenerative intervertebral discs: A proteomic analysis
Description	Mesenchymal stromal cells (MSCs) have been introduced as promising cell source for regenerative medicine. Besides their multilineage differentiation capacity, MSCs release a wide spectrum of bioactive factors. This secretome holds immunomodulatory and regenerative capacities. In intervertebral disc (IVD) cells, application of MSC secretome has been shown to decrease the apoptosis rate, induce proliferation and promote production of extracellular matrix (ECM). For clinical translation of secretome-based treatment, characterization of the secretome composition is needed to better understand the induced biological processes and identify potentially effective secretomes. Methods: This study aimed to investigate the proteome released by bone marrow derived MSCs following exposure to a healthy, traumatic, or degenerative human IVD environment by mass spectroscopy and quantitative immunoassay analyses. Exposure of MSCs to the proinflammatory stimulus interleukin 1β (IL-1b) was used as control.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_08:01:22.574.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Witold Wolski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-03 01:39:52	ID requested
⏵ 1	2021-09-09 08:01:23	announced

Wangler S, Kamali A, Wapp C, Wuertz-Kozak K, H, ä, ckel S, Fortes C, Benneker LM, Haglund L, Richards RG, Alini M, Peroglio M, Grad S, Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic, and degenerative intervertebral discs: a proteomic analysis. Stem Cell Res Ther, 12(1):11(2021) [pubmed]

submitter keyword: Human, Mesenchymal stromal cells, LC-MS, MS1 Quantification

Sibylle Grad
contact affiliation	Focus Area Leader Disc and Cartilage Biology, PhD, PD AO Research Institute Davos (ARI)
contact email	sibylle.grad@aofoundation.org
lab head
Witold Wolski
contact affiliation	Functional Genomics Center Zurch
contact email	wew@fgcz.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021281

PRIDE project URI

• PRIDE
  1. PXD021281
     1. Label: PRIDE project
     2. Name: Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic and 1 degenerative intervertebral discs: A proteomic analysis