PXD021227 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CSR-1A post-translational modification |
Description | CSR-1 is an essential Argonaute protein that binds to a subclass of 22G-RNAs targeting most germline-expressed genes. Here we show that the two isoforms of CSR-1 have distinct expression patterns; CSR-1B is ubiquitously expressed throughout the germline and during all stages of development while CSR-1A expression is restricted to germ cells undergoing spermatogenesis. Furthermore, CSR-1A associates preferentially with 22G-RNAs mapping to spermatogenesis-specific genes whereas CSR-1B-bound small RNAs map predominantly to oogenesis-specific genes. Interestingly, the exon unique to CSR-1A contains multiple dimethylarginine modifications, which are necessary for the preferential binding of CSR-1A to spermatogenesis-specific 22G-RNAs. Thus, we have discovered a regulatory mechanism for C. elegans Argonaute proteins that allows for specificity of small RNA binding between similar Argonaute proteins with overlapping temporal and spatial localization. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-20 |
AnnouncementXML | Submission_2021-07-20_02:35:04.889.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021227 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Carolyn Phillips |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | methylated residue; dimethylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-31 22:07:19 | ID requested | |
⏵ 1 | 2021-07-20 02:35:05 | announced | |
2 | 2021-07-20 02:35:46 | announced | 2021-07-20: Updated publication reference for PubMed record(s): 34244496. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: C. elegans, CSR-1A, dimethylation |
Contact List
Carolyn M. Phillips |
contact affiliation | Department of Biological Sciences, University of Southern California, Los Angeles CA |
contact email | cphil@usc.edu |
lab head | |
Carolyn Phillips |
contact affiliation | University of Southern California |
contact email | cphil@usc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021227
- Label: PRIDE project
- Name: CSR-1A post-translational modification