PXD021222-1

PXD021222 is an original dataset announced via ProteomeXchange.

Title	Inhibiting coronavirus replication by chemical ER stress, including SARS-CoV-2 and MERS-CoV
Description	Coronaviruses (CoVs) are important human pathogens for which no specific treatment is available. Here, we provide evidence that pharmacological reprogramming of ER stress pathways can be exploited to suppress CoV replication. We found that the ER stress inducer thapsigargin efficiently inhibits coronavirus (HCoV-229E, MERS-CoV, SARS-CoV-2) replication in different cell types, (partially) restores the virus-induced translational shut-down, and counteracts the CoV-mediated downregulation of IRE1α and the ER chaperone BiP. Proteome-wide data sets revealed specific pathways, protein networks and components that likely mediate the thapsigargin-induced antiviral state, including HERPUD1, an essential factor of ER quality control, and ER-associated protein degradation complexes. The data show that thapsigargin hits a central mechanism required for CoV replication, suggesting that thapsigargin (or derivatives thereof) may be developed into broad-spectrum anti-CoV drugs.
HostingRepository	PRIDE
AnnounceDate	2021-06-24
AnnouncementXML	Submission_2021-06-24_02:27:52.133.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021222
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Uwe Linne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049; scientific name: Middle East respiratory syndrome-related coronavirus; NCBI TaxID: 1335626; scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 04:07:38	ID requested
⏵ 1	2021-06-24 02:27:52	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: Coronavirus, MERS-CoV, SARS-CoV-2, HCoV-229E, Thapsigargin

Uwe Linne
contact affiliation	Head of Core Facility Mass Spectrometry, Faculty of Chemistry, Philipps-University Marburg
contact email	linneu@staff.uni-marburg.de
lab head
Uwe Linne
contact affiliation	Head of Core Facility Mass Spectrometry, Faculty of Chemistry, Philipps-University Marburg
contact email	linneu@staff.uni-marburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021222
     1. Label: PRIDE project
     2. Name: Inhibiting coronavirus replication by chemical ER stress, including SARS-CoV-2 and MERS-CoV