PXD021216 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Alkaloids modify the poison frog proteome |
Description | Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (Oophaga sylvatica) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinoline within four days, and dietary alkaloid exposure modified protein abundance in the intestines, liver, and skin. Many proteins that increased in abundance with toxin accumulation are plasma glycoproteins, including the complement system and the toxin-binding protein saxiphilin. Other protein classes that change in abundance with toxin accumulation are membrane proteins involved in small molecule transport and metabolism. Overall, this work shows poison frogs can rapidly accumulate alkaloids, which alter carrier protein abundance, initiate an immune response, and alter small molecule transport and metabolism dynamics across tissues |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-25 |
AnnouncementXML | Submission_2021-01-24_23:00:30.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021216 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jeremy OConnell |
SpeciesList | scientific name: Oophaga sylvatica; NCBI TaxID: 152496; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-31 01:54:12 | ID requested | |
⏵ 1 | 2021-01-24 23:00:30 | announced | |
Publication List
O'Connell LA, O'Connell JD, Paulo JA, Trauger SA, Gygi SP, Murray AW, Rapid toxin sequestration modifies poison frog physiology. J Exp Biol, 224(Pt 3):(2021) [pubmed] |
Keyword List
submitter keyword: Poison Dart Frog,gut, liver, skin, toxin, saxiphilin, complement system, cytochrome P450s |
Contact List
Lauren A. O’Connell |
contact affiliation | Department of Biology Stanford University 371 Jane Stanford Way Stanford, CA 94305 |
contact email | loconnel@stanford.edu |
lab head | |
Jeremy OConnell |
contact affiliation | Harvard Medical School |
contact email | theoconnell@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021216
- Label: PRIDE project
- Name: Alkaloids modify the poison frog proteome