PXD021194-1

PXD021194 is an original dataset announced via ProteomeXchange.

Title	Novel skin photoageing biomarkers of epidermis and dermis revealed by traditional relative quantification in combination with a novel peptide locational fingerprinting MPLF.
Description	Human skin undergoes profound structural remodelling following chronic exposure to ultraviolet radiation (UVR) – photoageing - that is normally studied by comparing photoexposed and photoprotected skin sites. Whilst it is well established that the abundance and distribution of major dermal extracellular matrix (ECM) proteins (principally collagen I and elastin) are compromised in skin, the influence of UVR exposure on other skin protein constituents, including epidermal proteins, is poorly understood. We used label-free LC-MS/MS proteomics to characterise the proteomic profiles of intrinsically aged buttock and photoaged forearm epidermis and dermis. Besides traditional shotgun proteomics we have also developed and used novel proteomics analysis approach to reveal locational changes in peptide yields from proteins named Manchester Peptide Locational Fingerprinting (MPLF) which is based on spectral counting. MPLF is a new proteomic analysis tool that can identify structural modification-associated differences within proteins from label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) datasets generated from biological samples. We have identified a total of 174 dermal proteins and 146 epidermal proteins with MPLF while relative quantification revealed a total of 635 dermal proteins and 926 epidermal proteins significantly different between photoaged forearm and intrinsically-aged buttock according to peak area intensity.
HostingRepository	PRIDE
AnnounceDate	2021-04-09
AnnouncementXML	Submission_2021-04-08_23:05:39.414.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021194
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Eckersley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-28 07:33:13	ID requested
⏵ 1	2021-04-08 23:08:07	announced

Ozols M, Eckersley A, Mellody KT, Mallikarjun V, Warwood S, O'Cualain R, Knight D, Watson REB, Griffiths CEM, Swift J, Sherratt MJ, Peptide location fingerprinting reveals modification-associated biomarker candidates of ageing in human tissue proteomes. Aging Cell, 20(5):e13355(2021) [pubmed]

submitter keyword: #skin #epidermis #dermis #biomarkers

Michael J Sherratt
contact affiliation	Affiliation: Division of Cell Matrix Biology & Regenerative Medicine, The University of Manchester [Data also submitted and owned by Dr Matiss Ozols (matiss.ozols@manchester.ac.uk)]
contact email	michael.j.sherratt@manchester.ac.uk
lab head
Alexander Eckersley
contact affiliation	University of Manchester
contact email	alexander.eckersley@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD021194

PRIDE project URI

• PRIDE
  1. PXD021194
     1. Label: PRIDE project
     2. Name: Novel skin photoageing biomarkers of epidermis and dermis revealed by traditional relative quantification in combination with a novel peptide locational fingerprinting MPLF.