PXD021173-1

PXD021173 is an original dataset announced via ProteomeXchange.

Title	The mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress
Description	Most mitochondrial proteins are synthesized as precursor proteins in the cytosol and post-translationally transported into mitochondria. The mitochondrial surface protein Tom70 acts at the interface of the cytosol and mitochondria. In vitro import experiments identified Tom70 as targeting receptor, particularly for hydrophobic carriers. Using in vivo methods, we revisited the Tom70 function, confirming that it supports the biogenesis of carriers and other mitochondrial proteins which considerably expands the set of Tom70-dependent mitochondrial proteins. However, the crucial activity of Tom70 is its recruitment of cytosolic chaperones to the outer membrane. Surprisingly, tethering an unrelated chaperone-binding domain onto the mitochondrial surface complements most of the defects caused by the absence of Tom70. In vivo, small single spanning inner membrane proteins can be particularly problematic when present in excess and the chaperone-binding ability of Tom70 is crucial to suppress their proteotoxicity. Thus, the predominant function of Tom70 is more that of a cytosolic co-chaperone, and its role as mitochondria-specifying receptor apparently is of less immediate relevance.
HostingRepository	PRIDE
AnnounceDate	2021-03-29
AnnouncementXML	Submission_2021-03-28_22:48:36.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robin Roth
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-27 06:54:49	ID requested
⏵ 1	2021-03-28 22:48:37	announced

Dataset with its publication pending

submitter keyword: Chaperones

Mitochondria

Outer membrane

Proteostasis

Tom70

Zuzana Storchova
contact affiliation	Technische Universität Kaiserslautern
contact email	storchova@bio.uni-kl.de
lab head
Robin Roth
contact affiliation	Technische Universität Kaiserslautern
contact email	rroth@bio.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD021173

PRIDE project URI

• PRIDE
  1. PXD021173
     1. Label: PRIDE project
     2. Name: The mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress