PXD021148-1

PXD021148 is an original dataset announced via ProteomeXchange.

Title	IP-MS Analysis of ESX-5 and ESX-1 Substrates Enables Mycobacterial Species Identification
Description	Pulmonary disease resulting from non-tuberculous mycobacteria (NTM) infection has emerged as an increasingly prevalent clinical entity in the past two to three decades, but there are no standardized, commercial assays available for the molecular diagnosis of NTM infections from clinical samples. Herein we discuss the development of an assay that employs immunoprecipitation coupled with mass spectrometry (IP-MS) to rapidly and accurately discriminate prevalent slow-growing mycobacterial species (i.e., M. avium and M. intracellulare, M. kansasii, M. gordonae, M. marinum and M. tuberculosis) during early growth in mycobacterial growth indicator tube (MGIT) cultures. This IP-MS assay employs antibodies specific for conserved tryptic peptides of M. tuberculosis EsxN (AQAASLEAEHQAIVR) and CFP-10 (TQIDQVESTAGSLQGQWR) to capture and identify specific mycobacterial species and allows species-specific mycobacterium identification at the first sign of MGIT culture positivity.
HostingRepository	PanoramaPublic
AnnounceDate	2022-04-18
AnnouncementXML	Submission_2022-04-18_20:47:41.830.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Qingbo Shu
SpeciesList	scientific name: Corynebacteriales; NCBI TaxID: 85007;
ModificationList	Label:13C(6)15N(4)
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 14:31:26	ID requested
⏵ 1	2022-04-18 20:47:42	announced

Shu Q, Rajagopal M, Fan J, Zhan L, Kong X, He Y, Rotcheewaphan S, Lyon CJ, Sha W, Zelazny AM, Hu T. Peptidomic analysis of mycobacterial secreted proteins enables species identification. VIEW [Internet]. 2022 Apr 18;n/a(n/a):20210019. Available from: https://doi.org/10.1002/VIW.20210019

submitter keyword: EsxN, IP-MS, Mycobacterium, species discrimination, tryptic peptides

Tony Hu
contact affiliation	Tulane Univerity
contact email	tonyhu@tulane.edu
lab head
Qingbo Shu
contact affiliation	Tulane University
contact email	qshu@tulane.edu
dataset submitter

Panorama Public dataset URI