PXD021124 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of IgE reactive proteins from Aspergillus terreus using mass spectrometry. |
Description | Aspergillus terreus has been described as an allergenic fungus in addition to causing infection to both human beings and plants. However, the allergens in this fungus are still unknown. The identification of allergens is essential to develop diagnostic and therapeutic avenues. Employing a proteomic approach, sixteen allergens were identified based on two-dimensional immuno-blotting with A. terreus susceptible patient sera. Amongst them, triosephosphate isomerase (Asp t 36), one of the dominant immunoglobulin E (IgE)-reactive proteins, was purified under native conditions. To characterize Asp t 36, the gene was cloned and expressed in E. coli. Phylogenetic analysis showed it to be highly conserved with close similarity to Dermatophagoides farinae. Four immuno-dominant epitopes were identified using synthetic peptides and mapped on the tertiary structure of Asp t 36. Among these, two were found to create a continuous surface patch on the 3D structure rendering it to be an IgE binding hotspot. Asp t 36 showed similar secondary structure and temperature sensitivity with other reported triosephosphate isomerase allergens. In-vivo studies using the murine model demonstrated rAsp t 36 able to stimulate airway inflammation, such as an influx of eosinophils, goblet cell hyperplasia, elevated serum immunoglobulins and induction of Th2 cytokines. Collectively, our results reveal the immunogenic property of Asp t 36, a major allergen from A. terreus for the first time in any fungi. This allergen could serve as a potent candidate for investigating component resolved diagnosis and immunotherapy. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-20 |
AnnouncementXML | Submission_2020-10-20_00:54:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bijoya Karmakar |
SpeciesList | scientific name: Aspergillus terreus NIH2624; NCBI TaxID: 341663; |
ModificationList | iodoacetamide derivatized residue |
Instrument | autoflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-26 00:04:40 | ID requested | |
⏵ 1 | 2020-10-20 00:54:34 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Aspergillus terreus, allergen, triosephosphate isomerase, proteomics, chromatography, circular dichroism, epitope mapping, airway inflammation, immunoglobulin E (IgE), immunoglobulin G (IgG). |
Contact List
Bijoya Karmakar and Swati Gupta Bhattacharya |
contact affiliation | Bijoya Karmakar, Division of Plant Biology, Bose Institute, Kolkata, India. Swati Gupta Bhattacharya, Professor, Division of Plant Biology Bose Institute 93/1 Acharya Prafulla Chandra Road, Kolkata 700009 |
contact email | swati@jcbose.ac.in |
lab head | |
Bijoya Karmakar |
contact affiliation | Senior Research Fellow |
contact email | bijoyak5@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021124
- Label: PRIDE project
- Name: Identification of IgE reactive proteins from Aspergillus terreus using mass spectrometry.