PXD021123 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | NCAM1: A Novel Autoantigen in Membranous Lupus Nephritis |
Description | Membranous lupus nephritis is a frequent cause of nephrotic syndrome in patients with systemic lupus erythematosus. Unlike phospholipase A2 receptor or thrombospondin type 1 domain containing 7A-associated membranous nephropathy, where known antibodies can be detected within sera by indirect immunofluorescence and/or enzyme-linked immunosorbent assay, it is not possible to monitor disease activity in membranous lupus nephritis where the target autoantigens are mostly unknown. Determination of the target autoantigen has diagnostic significance, informs prognosis, and allows for non-invasive monitoring of disease activity in serum. We utilized mass spectrometry for antigen discovery of laser capture microdissected glomeruli from formalin-fixed paraffin embedded tissue and tissue IgG immunoprecipitation studies from frozen kidney biopsy tissue. We identified neural cell adhesion molecule 1 (NCAM1) to be a target antigen in membranous lupus nephritis and within rare cases of primary membranous nephropathy. The prevalence of NCAM1-associated membranous neuropathy was 5.7% of cases of membranous lupus nephritis. NCAM1 co-localizes with IgG within glomerular immune deposits. Additionally, serum from NCAM1 patients showed reactivity to NCAM1 recombinant protein. The presence of anti-NCAM1 antibodies in sera could allow for non-invasive monitoring of the disease. We propose that NCAM1 is a target autoantigen in a subset of patients with membranous lupus nephritis. Future studies are needed to determine whether anti-NCAM1 antibody levels correlate with disease activity or response to therapy. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-19 |
AnnouncementXML | Submission_2020-10-19_01:09:45.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021123 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Aaron Storey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-26 00:01:04 | ID requested | |
⏵ 1 | 2020-10-19 01:09:46 | announced | |
Publication List
Caza TN, Hassen SI, Kuperman M, Sharma SG, Dvanajscak Z, Arthur J, Edmondson R, Storey A, Herzog C, Kenan DJ, Larsen CP, Neural cell adhesion molecule 1 is a novel autoantigen in membranous lupus nephritis. Kidney Int, 100(1):171-181(2021) [pubmed] |
Keyword List
submitter keyword: Human, Kidney, DDA |
Contact List
Chris Larsen |
contact affiliation | Arkana Laboratories, Little Rock, Arkansas, USA |
contact email | chris.larsen@arkanalabs.com |
lab head | |
Aaron Storey |
contact affiliation | University of Arkansas for Medical Sciences |
contact email | ajstorey@uams.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021123
- Label: PRIDE project
- Name: NCAM1: A Novel Autoantigen in Membranous Lupus Nephritis