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PXD021039-1

PXD021039 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry runs and analysis of host proteins co-immunoprecipitated with RABV M protein
DescriptionTo explore the role of the matrix protein in RABV infection of host cells, we looked for host proteins that interact with the RABV M protein. We performed Flag-tag-based immunoprecipitation of lysates of HEK293T cells transfected with plasmids expressing either N- or C-terminally Flag-tagged M protein (M-NF or M-CF, respectively), and identified host proteins that interacted with the M proteins by using mass spectrometry (MS). Proteins were detected in lysates of cells transfected with the plasmid expressing Flag-tagged M proteins but not in those of cells transfected with the control plasmid. We identified a total of 2,672 host proteins that co-immunoprecipitated with the M protein; 492 proteins co-immunoprecipitated to both M-NF and M-CF, 1,761 proteins co-immunoprecipitated with M-NF only, and 419 proteins co-immunoprecipitated with M-CF only.
HostingRepositoryPRIDE
AnnounceDate2021-09-09
AnnouncementXMLSubmission_2021-09-09_07:16:15.351.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXing Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-20 23:37:08ID requested
12021-09-09 07:16:16announced
Publication List
Liu X, Li F, Zhang J, Wang L, Wang J, Wen Z, Wang Z, Shuai L, Wang X, Ge J, Zhao D, Bu Z, The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein. J Biol Chem, 296():100096(2021) [pubmed]
Keyword List
submitter keyword: Mass spectrometry, host proteins, co-immunoprecipitation, RABV, matrix protein
Contact List
Zhigao Bu
contact affiliationChinese Academy of Agricultural Sciences, State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, China
contact emailbuzhigao@caas.cn
lab head
Xing Liu
contact affiliationChinese Academy of Agricultural Sciences
contact emailliuxingshenglin@163.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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