PXD021039-1

PXD021039 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry runs and analysis of host proteins co-immunoprecipitated with RABV M protein
Description	To explore the role of the matrix protein in RABV infection of host cells, we looked for host proteins that interact with the RABV M protein. We performed Flag-tag-based immunoprecipitation of lysates of HEK293T cells transfected with plasmids expressing either N- or C-terminally Flag-tagged M protein (M-NF or M-CF, respectively), and identified host proteins that interacted with the M proteins by using mass spectrometry (MS). Proteins were detected in lysates of cells transfected with the plasmid expressing Flag-tagged M proteins but not in those of cells transfected with the control plasmid. We identified a total of 2,672 host proteins that co-immunoprecipitated with the M protein; 492 proteins co-immunoprecipitated to both M-NF and M-CF, 1,761 proteins co-immunoprecipitated with M-NF only, and 419 proteins co-immunoprecipitated with M-CF only.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_07:16:15.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xing Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-20 23:37:08	ID requested
⏵ 1	2021-09-09 07:16:16	announced

Liu X, Li F, Zhang J, Wang L, Wang J, Wen Z, Wang Z, Shuai L, Wang X, Ge J, Zhao D, Bu Z, The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein. J Biol Chem, 296():100096(2021) [pubmed]

submitter keyword: Mass spectrometry, host proteins, co-immunoprecipitation, RABV, matrix protein

Zhigao Bu
contact affiliation	Chinese Academy of Agricultural Sciences, State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, China
contact email	buzhigao@caas.cn
lab head
Xing Liu
contact affiliation	Chinese Academy of Agricultural Sciences
contact email	liuxingshenglin@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021039
     1. Label: PRIDE project
     2. Name: Mass spectrometry runs and analysis of host proteins co-immunoprecipitated with RABV M protein