PXD021039 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry runs and analysis of host proteins co-immunoprecipitated with RABV M protein |
Description | To explore the role of the matrix protein in RABV infection of host cells, we looked for host proteins that interact with the RABV M protein. We performed Flag-tag-based immunoprecipitation of lysates of HEK293T cells transfected with plasmids expressing either N- or C-terminally Flag-tagged M protein (M-NF or M-CF, respectively), and identified host proteins that interacted with the M proteins by using mass spectrometry (MS). Proteins were detected in lysates of cells transfected with the plasmid expressing Flag-tagged M proteins but not in those of cells transfected with the control plasmid. We identified a total of 2,672 host proteins that co-immunoprecipitated with the M protein; 492 proteins co-immunoprecipitated to both M-NF and M-CF, 1,761 proteins co-immunoprecipitated with M-NF only, and 419 proteins co-immunoprecipitated with M-CF only. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_07:16:15.351.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xing Liu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-20 23:37:08 | ID requested | |
⏵ 1 | 2021-09-09 07:16:16 | announced | |
Publication List
Liu X, Li F, Zhang J, Wang L, Wang J, Wen Z, Wang Z, Shuai L, Wang X, Ge J, Zhao D, Bu Z, The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein. J Biol Chem, 296():100096(2021) [pubmed] |
Keyword List
submitter keyword: Mass spectrometry, host proteins, co-immunoprecipitation, RABV, matrix protein |
Contact List
Zhigao Bu |
contact affiliation | Chinese Academy of Agricultural Sciences, State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, China |
contact email | buzhigao@caas.cn |
lab head | |
Xing Liu |
contact affiliation | Chinese Academy of Agricultural Sciences |
contact email | liuxingshenglin@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021039
- Label: PRIDE project
- Name: Mass spectrometry runs and analysis of host proteins co-immunoprecipitated with RABV M protein