PXD021034-1

PXD021034 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria
Description	Cells harbor two systems for fatty acid synthesis, one in the cytoplasm (catalyzed by fatty acid synthase, FASN) and one in the mitochondria (mtFAS). In contrast to FASN, mtFAS is poorly characterized, especially in higher eukaryotes, with the major product(s), metabolic roles, and cellular function(s) being essentially unknown. Here we show that hypomorphic mtFAS mutant mouse skeletal myoblast cell lines display a severe loss of electron transport chain (ETC) complexes and exhibit compensatory metabolic activities including reductive carboxylation. This effect on ETC complexes appears to be independent of protein lipoylation, the best characterized function of mtFAS, as mutants lacking lipoylation have an intact ETC. Finally, mtFAS impairment blocks the differentiation of skeletal myoblasts in vitro. Together, these data suggest that ETC activity in mammals is profoundly controlled by mtFAS function, thereby connecting anabolic fatty acid synthesis with the oxidation of carbon fuels.
HostingRepository	PRIDE
AnnounceDate	2020-09-02
AnnouncementXML	Submission_2020-09-02_07:07:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-20 22:54:46	ID requested
⏵ 1	2020-09-02 07:07:24	announced

Nowinski SM, Solmonson A, Rusin SF, Maschek JA, Bensard CL, Fogarty S, Jeong MY, Lettlova S, Berg JA, Morgan JT, Ouyang Y, Naylor BC, Paulo JA, Funai K, Cox JE, Gygi SP, Winge DR, DeBerardinis RJ, Rutter J, Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. Elife, 9():(2020) [pubmed]

submitter keyword: Mitochondria, fatty acid synthesis, electron transport chain, reductive carboxylation, lipoylation TCA cycle, metabolism, respiration, OXPHOS, muscle

Steven P. Gygi
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston, MA, USA
contact email	sgygi@hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021034
     1. Label: PRIDE project
     2. Name: Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria