PXD021029-1

PXD021029 is an original dataset announced via ProteomeXchange.

Title	Dissection of anti-tumor activity of histone deacetylase inhibitor SAHA in NPC cells via quantitative phosphoproteomics
Description	Suberoylanilide hydroxamic acid (SAHA), as a pan HDAC inhibitor, has been approved for clinical use and it has been demonstrated to treat solid tumor. Nevertheless, the mechanism underlying the therapeutic effects of SAHA on tumors are still not fully understood. Protein phosphorylation is one of the most important regulatory events in cells, regulating primary biological processes, such as cell division, growth, migration, differentiation, and intercellular communication. The impact of SAHA treatment on cellular phosphorylation and signaling pathways might contribute to our understanding on its therapeutic mechanism. For this purpose, by combining tandem mass tags(TMT)-based quantitative proteomics and titanium dioxide-based phosphopeptide enrichment, we for the first time comprehensively identified and quantified protein phosphorylation in NPC cells toward SAHA treatment. we identified 7430 phosphorylation sites on 2456 phosphoproteins in the NPC cell line 5-8F, of which 1176 phosphorylation sites on 528 phosphoproteins were significantly expressed upon SAHA treatment. Biological analysis show that SAHA can influence several biological processes including mRNA/DNA processing, cell cycle. Furthermore, signaling pathway analysis demonstrate that SAHA can activate cell cycle regulation related pathway such as p53 signalling pathway, and inactivate energetic pathway such as AMPK pathway etc. Overall, our study presents evidence at a phosphoproteomic level that SAHA can inhibit NPC cell growth by regulating the cell cycle and cell energy metabolism. Our findings will contribute to elucidation of the mechanisms through which SAHA exerts its anticancer effects.
HostingRepository	iProX
AnnounceDate	2020-08-20
AnnouncementXML	Submission_2020-08-20_19:31:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maoyu Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-20 19:29:38	ID requested
⏵ 1	2020-08-20 19:31:20	announced

Dataset with its publication pending

submitter keyword: HDAC inhibitor, SAHA, quantitative phosphoproteomics

Yongheng Chen
contact affiliation	NHC Key Lab of Cancer Proteomics, Xiangya Hospital, Central South University
contact email	yonghenc@163.com
lab head
Maoyu Li
contact affiliation	Central South University
contact email	maoyuli@126.com
dataset submitter

iProX dataset URI