PXD021026-1

PXD021026 is an original dataset announced via ProteomeXchange.

Title	Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiation
Description	Bone marrow-derived macrophages (BMDMs) are a key model system for studying macrophage biology in vitro. Commonly used methods to differentiate macrophages from bone marrow are treatment with either recombinant M-CSF or the supernatant of L929 cells, which secrete M-CSF. However, little is known about the composition of L929 conditioned media (CM) and how it affects BMDM phenotype. Here, we used quantitative mass spectrometry to characterise the kinetics of protein secretion from L929 cells over a two-week period. While M-CSF is very abundant in L929 CM, we identified several other immune-regulatory proteins at surprisingly high abundance. L929 CM induced a slightly pre-activated phenotype and the expression of a number of known innate immune proteins in macrophages. This resource will be valuable to all researchers using L929 CM for the differentiation of BMDMs.
HostingRepository	PRIDE
AnnounceDate	2021-04-13
AnnouncementXML	Submission_2021-04-13_06:53:49.113.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-20 08:42:08	ID requested
⏵ 1	2021-04-13 06:53:49	announced

Dataset with its publication pending

submitter keyword: Bone marrow-derived macrophages, L929, secretome, proteomics, differentiation, M-CSF

Matthias Trost
contact affiliation	Newcastle University Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE24HH, United Kingdom
contact email	matthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD021026

PRIDE project URI

• PRIDE
  1. PXD021026
     1. Label: PRIDE project
     2. Name: Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiation