PXD021021 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Altered turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35 |
Description | Fatty acid 2-hydroxylase (FA2H) is responsible for the synthesis of 2-hydroxylated fatty acids, which are a building block of sphingolipids. This 2-hydroxylated sphingolipids can be found in various tissues such as brain, spinal cord, skin, testis, ovary, kidney, stomach, or intestine with a notably high occurrence in the insulating myelin sheath of neurons. Mutations in the FA2H gene can cause the neurodegenerative disease spastic paraplegia 35 (SPG35), which is also known as fatty acid hydroxylase-associated neurodegeneration. Moreover, FA2H knock-out mice largely resemble the SPG35 human disease phenotype. To further elucidate the connection between FA2H and SPG35, we performed a comparative quantitative proteome analysis of central nervous system myelin of wildtype and FA2H-KO mice at different ages (6, 13, and 17 months) using isobaric labeling with tandem mass tags (TMT). |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-04 |
AnnouncementXML | Submission_2021-03-04_13:44:57.042.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Hardt |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-20 04:08:38 | ID requested | |
⏵ 1 | 2021-03-04 13:44:57 | announced | |
Publication List
Hardt R, Jordans S, Winter D, Gieselmann V, Wang-Eckhardt L, Eckhardt M, Decreased turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35. Hum Mol Genet, 29(22):3616-3630(2021) [pubmed] |
Keyword List
submitter keyword: Isobaric labeling, TMT, myelin, CNS, mice, fatty acid hydroxylation, sphingolipids, fatty acid 2-hydroxylase, FA2H, neurodegeneration, disease model, time course, hereditary spastic paraplegia 35, Opalin |
Contact List
Matthias Eckhardt |
contact affiliation | AK Gieselmann Institut für Biochemie und Molekularbiologie (IBMB) Med. Fak. Universtität Bonn Nussallee 11 53115 Bonn Germany |
contact email | eckhardt@uni-bonn.de |
lab head | |
Robert Hardt |
contact affiliation | IBMB - University of Bonn |
contact email | rhardt@uni-bonn.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021021
- Label: PRIDE project
- Name: Altered turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35