PXD020982 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MS/MS analysis of Ub-conjugated UbcH7 ubiquitination sites |
Description | By conducting the ubiquitin-conjugation assay for UbcH7, we found that PknG was able to promote the loading of Ub onto UbcH7, which is indicated by a molecular weight shift in SDS–PAGE. The classic Ub conjugation of E2s requires the E1 to form a thiol-ester bond between its active Cys and the C-terminal Gly of Ub. Notably, PknG could directly catalyze Ub conjugation onto UbcH7 in a kinase activity-independent and Ubl domain-dependent manner. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-16 |
AnnouncementXML | Submission_2021-04-16_03:35:01.253.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | lu z |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-18 22:32:11 | ID requested | |
⏵ 1 | 2021-04-16 03:35:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: MS, unconventional isopeptidase activitys |
Contact List
Cuihua Liu |
contact affiliation | CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; |
contact email | wangj6@im.ac.cn |
lab head | |
lu z |
contact affiliation | Institute of Microbiology |
contact email | lu1457883143@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD020982 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020982
- Label: PRIDE project
- Name: MS/MS analysis of Ub-conjugated UbcH7 ubiquitination sites