PXD020951-1

PXD020951 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis demonstrated transcription factor gene YRR1 deletion in Saccharomyces cerevisiae enhances its resistance to vanillin through the upregulation of transcriptional activator Haa1, coactivator Mbf1, and proteasome assembly chaperone Tma17 expression
Description	Vanillin is one of the major phenolic inhibitors of Saccharomyces cerevisiae in lignocellulosic hydrolysates. Deleting transcription factor gene YRR1 improves vanillin resistance by promoting some translation-related processes that were confirmed at the transcription level in our previous studies. In this work, we investigated the effect of proteomic change on vanillin stress and YRR1 deletion. In wild-type cells, vanillin reduced the number of ribosomal proteins and thereby inhibited cells’ translation. YRR1 deletion increased 112 protein quantities; 48 of 112 up-regulated proteins are involved in the stress response, translational and basal transcriptional regulation. Fermentation data showed that the overexpression of HAA1, MBF1, and TMA17, which encode transcriptional activator, coactivator, and proteasome assembly chaperone, respectively, enhanced resistance to vanillin in S. cerevisiae. These results enriched the perspective of molecular mechanisms for YRR1 deletion to protect yeast from vanillin stress and offered novel targets for designing inhibitor-resistant ethanologenic yeast strains.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:44:44.902.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinning Wang
SpeciesList	scientific name: Saccharomyces cf. cerevisiae; NCBI TaxID: 2069377;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-17 03:34:38	ID requested
⏵ 1	2021-09-09 02:44:45	announced

Cao W, Zhao W, Yang B, Wang X, Shen Y, Wei T, Qin W, Li Z, Bao X, Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae. Microb Cell Fact, 20(1):142(2021) [pubmed]

submitter keyword: YRR1

quantitative proteomics

vanillin resistance

bioethanol

Saccharomyces cerevisiae

Xinning Wang
contact affiliation	School of Biotechnology, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China；
contact email	xnwang@qlu.edu.cn
lab head
Xinning Wang
contact affiliation	Qilu University of Technology, Shandong Academy of Sciences
contact email	xnwang@qlu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020951
     1. Label: PRIDE project
     2. Name: Proteomic analysis demonstrated transcription factor gene YRR1 deletion in Saccharomyces cerevisiae enhances its resistance to vanillin through the upregulation of transcriptional activator Haa1, coactivator Mbf1, and proteasome assembly chaperone Tma17 expression