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PXD020946-1

PXD020946 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEC-exosomes and BM fluid-Exosomes
DescriptionWe isolated the exosomes from mouse bone marrow fluid, mouse primary bone marrow endothelial cells (BMEC) culture supernatants of WT-BMEC and Vps33b knockdown BMECs by ultracentrifugation. After characterization of isolated exosomes, A global comparison of the protein cargo carried by the different exosomes. In brief,protein were extracted and labeled with TMT, the peptide were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in a Q Exactive HF-X (Thermo) coupled with an EASY-nLC 1200 UPLC system, and the resulting MS/MS data were processed using the MaxQuant search engine.
HostingRepositoryPRIDE
AnnounceDate2020-11-24
AnnouncementXMLSubmission_2020-11-23_23:20:25.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuohuan Sun
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-17 03:08:34ID requested
12020-11-23 23:20:26announced
Publication List
Huang D, Sun G, Hao X, He X, Zheng Z, Chen C, Yu Z, Xie L, Ma S, Liu L, Zhou BO, Cheng H, Zheng J, Cheng T, ANGPTL2-containing small extracellular vesicles from vascular endothelial cells accelerate leukemia progression. J Clin Invest, 131(1):(2021) [pubmed]
Keyword List
submitter keyword: EC-exosomes
BM fluid-Exosomes
Contact List
Guohuan Sun
contact affiliationState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
contact emailsunguohuan@ihcams.ac.cn
lab head
Guohuan Sun
contact affiliationState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
contact emailsunguohuan@ihcams.ac.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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