PXD020930-1

PXD020930 is an original dataset announced via ProteomeXchange.

Title	Multiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues
Description	We developed 6,927 quantitative multiple reaction monitoring mass spectrometry (MRM-MS) assays that measure 2,187 unique proteins in 20 mouse organs and tissues to enable system-level analysis of protein expression in mice. Targets for assay development were selected by untargeted MS analysis, and corresponding stable isotope-labelled standards were synthesized in-house. Development experiments determined the lower limit of quantitation, linear range, and repeatability of each assay. Subsequently, the developed assays were formatted into panels tailored to each organ or tissue type and were used to measure normal protein concentrations in samples from three mouse strains; C57BL/6NCrl, NOD/SCID, and BALB/cAnNCrl. In total 5,149 measurements were obtained corresponding to 1,691 unique proteins, and results included sex- and strain-specific profiles in protein abundances. Collectively, these assays are the largest group of quantitative MRM-MS assays established and implemented in mice to date, and enable accurate quantitation of mouse proteins for molecular phenotyping. Furthermore, the measured concentration data provide important biological information for experimental design involving mouse models.
HostingRepository	PanoramaPublic
AnnounceDate	2024-01-04
AnnouncementXML	Submission_2024-01-04_14:12:28.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Michaud
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	6495B Triple Quadrupole LC/MS; 6495A Triple Quadrupole LC/MS; 6490 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-16 21:47:16	ID requested
⏵ 1	2024-01-04 14:12:29	announced

Michaud SA, P, ě, tro, š, ov, á H, Sinclair NJ, Kinnear AL, Jackson AM, McGuire JC, Hardie DB, Bhowmick P, Ganguly M, Flenniken AM, Nutter LMJ, McKerlie C, Smith D, Mohammed Y, Schibli D, Sickmann A, Borchers CH, Multiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues. Commun Biol, 7(1):6(2024) [pubmed]

submitter keyword: Mouse tissue proteomics, MRM assay development, CPTAC, stable isotope-labelled standards, proteomic phenotyping, molecular phenotyping

Sarah Michaud
contact affiliation	U Victoria - Genome BC Proteomics Centre
contact email	sarah@proteincentre.com
lab head
Sarah Michaud
contact affiliation	U Victoria - Genome BC Proteomics Centre
contact email	sarah@proteincentre.com
dataset submitter

Panorama Public dataset URI