PXD020924-1

PXD020924 is an original dataset announced via ProteomeXchange.

Title	Different research approaches in unraveling the venom proteome of Naja ashei
Description	The dynamic development of venomics in recent years has resulted in a significant increase in publicly available proteomic data. The information contained therein is often used for comparisons between different datasets and to draw biological conclusions therefrom. We aimed to show the possible differences that can arise, in the final results of the proteomic experiment, while using different research workflows. We applied two software solutions (Peptide Shaker and MaxQuant) to process data from shotgun LC-MS/MS analysis of Naja ashei venom and collate it with the previous report concerning this species. Our results underline the necessary caution in the interpretation of data based on a comparative analysis of data derived from different studies.
HostingRepository	PRIDE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-07_05:37:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020924
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Konrad Hus
SpeciesList	scientific name: Naja ashei; NCBI TaxID: 696811;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-15 16:13:59	ID requested
⏵ 1	2020-09-07 05:37:12	announced

Dataset with its publication pending

submitter keyword: snake venom, venomics, absolute protein quantification, label-free shotgun mass spectrometry

Aleksandra Bocian
contact affiliation	Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland
contact email	bocian@prz.edu.pl
lab head
Konrad Hus
contact affiliation	Rzeszow University of Technology
contact email	knr.hus@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020924

PRIDE project URI

• PRIDE
  1. PXD020924
     1. Label: PRIDE project
     2. Name: Different research approaches in unraveling the venom proteome of Naja ashei