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PXD020916 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSelective dephosphorylation by PP2A-B55 directs the meiosis I - meiosis II transition in oocytes
DescriptionMeiosis is a specialized cell cycle that requires sequential changes to the cell division machinery to facilitate changing functions. To define the mechanisms that enable the oocyte-to-embryo transition, we performed time-course proteomics in sea star oocytes from prophase I through the first embryonic cleavage. Although protein levels are broadly stable, dynamic waves of phosphorylation underlie each meiotic stage. We find that the phosphatase PP2A-B55 is reactivated at the Meiosis I/II transition resulting in the preferential dephosphorylation of threonine residues. Selective dephosphorylation is critical for directing the MI / MII transition as altering PP2A-B55 substrate preferences disrupts key cell cycle events after meiosis I. In addition, threonine to serine substitution of a conserved phosphorylation site in the substrate INCENP prevents its relocalization at anaphase I. Thus, through its inherent phospho-threonine preference, PP2A-B55 imposes specific phosphoregulated behaviors that distinguish the two meiotic divisions.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMark Adamo
SpeciesList scientific name: Patiria miniata; common name: bat star; NCBI TaxID: 46514;
ModificationListTMT6plex; Carbamidomethyl; Oxidation; Phospho
InstrumentOrbitrap Fusion; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-14 12:45:34ID requested
12021-08-12 13:05:56announced
Publication List
no publication
Keyword List
submitter keyword: starfish, sea star, gamete, meiosis, oocyte, prophase, PP1, PP2A, PP2A-B55, protein phosphatase, phosphorylation, dephosphorylation, INCENP, TMT
Contact List
Arminja Kettenbach
contact affiliationThe Geisel School of Medicine at Dartmouth
contact emailarminja.n.kettenbach@dartmouth.edu
lab head
Mark Adamo
contact affiliationDartmouth College
contact emailmark.e.adamo@dartmouth.edu
dataset submitter
Full Dataset Link List
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Dataset FTP location
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