<?xml version="1.0" encoding="UTF-8" standalone="yes"?> <ProteomeXchangeDataset xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" formatVersion="1.4.0" id="PXD020916-1" xsi:noNamespaceSchemaLocation="proteomeXchange-1.4.0.xsd"> <CvList> <Cv fullName="PSI-MS" id="MS" uri="http://psidev.cvs.sourceforge.net/viewvc/*checkout*/psidev/psi/psi-ms/mzML/controlledVocabulary/psi-ms.obo"/> <Cv fullName="UNIMOD" id="UNIMOD" uri="http://www.unimod.org/obo/unimod.obo"/> <Cv fullName="PSI-MOD" id="MOD" uri="http://psidev.cvs.sourceforge.net/psidev/psi/mod/data/PSI-MOD.obo"/> </CvList> <DatasetSummary announceDate="2021-08-12" hostingRepository="MassIVE" title="Selective dephosphorylation by PP2A-B55 directs the meiosis I - meiosis II transition in oocytes"> <Description><![CDATA[Meiosis is a specialized cell cycle that requires sequential changes to the cell division machinery to facilitate changing functions. To define the mechanisms that enable the oocyte-to-embryo transition, we performed time-course proteomics in sea star oocytes from prophase I through the first embryonic cleavage. Although protein levels are broadly stable, dynamic waves of phosphorylation underlie each meiotic stage. We find that the phosphatase PP2A-B55 is reactivated at the Meiosis I/II transition resulting in the preferential dephosphorylation of threonine residues. Selective dephosphorylation is critical for directing the MI / MII transition as altering PP2A-B55 substrate preferences disrupts key cell cycle events after meiosis I. In addition, threonine to serine substitution of a conserved phosphorylation site in the substrate INCENP prevents its relocalization at anaphase I. Thus, through its inherent phospho-threonine preference, PP2A-B55 imposes specific phosphoregulated behaviors that distinguish the two meiotic divisions.]]></Description> <ReviewLevel> <cvParam accession="MS:1002855" cvRef="MS" name="Non peer-reviewed dataset"/> </ReviewLevel> <RepositorySupport> <cvParam accession="MS:1002856" cvRef="MS" name="Supported dataset by repository"/> </RepositorySupport> </DatasetSummary> <DatasetIdentifierList> <DatasetIdentifier> <cvParam accession="MS:1002487" cvRef="MS" name="MassIVE dataset identifier" value="MSV000085949"/> <cvParam accession="MS:1001919" cvRef="MS" name="ProteomeXchange accession number" value="PXD020916"/> <cvParam accession="MS:1001921" cvRef="MS" name="ProteomeXchange accession number version number" value="1"/> </DatasetIdentifier> </DatasetIdentifierList> <DatasetOriginList> <DatasetOrigin> <cvParam accession="MS:1002868" cvRef="MS" name="Original data"/> </DatasetOrigin> </DatasetOriginList> <SpeciesList> <Species> <cvParam accession="MS:1001469" cvRef="MS" name="taxonomy: scientific name" value="Patiria miniata"/> <cvParam accession="MS:1001468" cvRef="MS" name="taxonomy: common name" value="bat star"/> <cvParam accession="MS:1001467" cvRef="MS" name="taxonomy: NCBI TaxID" value="46514"/> </Species> </SpeciesList> <InstrumentList> <Instrument id="Instrument_1"> <cvParam accession="MS:1002416" cvRef="MS" name="Orbitrap Fusion"/> </Instrument> <Instrument id="Instrument_2"> <cvParam accession="MS:1002732" cvRef="MS" name="Orbitrap Fusion Lumos"/> </Instrument> </InstrumentList> <ModificationList> <cvParam accession="UNIMOD:737" cvRef="UNIMOD" name="TMT6plex"/> <cvParam accession="UNIMOD:4" cvRef="UNIMOD" name="Carbamidomethyl"/> <cvParam accession="UNIMOD:35" cvRef="UNIMOD" name="Oxidation"/> <cvParam accession="UNIMOD:21" cvRef="UNIMOD" name="Phospho"/> </ModificationList> <ContactList> <Contact id="project_submitter"> <cvParam accession="MS:1002037" cvRef="MS" name="dataset submitter"/> <cvParam accession="MS:1000586" cvRef="MS" name="contact name" value="Mark Adamo"/> <cvParam accession="MS:1000589" cvRef="MS" name="contact email" value="mark.e.adamo@dartmouth.edu"/> <cvParam accession="MS:1000590" cvRef="MS" name="contact affiliation" value="Dartmouth College"/> </Contact> <Contact id="principal_investigator"> <cvParam accession="MS:1002332" cvRef="MS" name="lab head"/> <cvParam accession="MS:1000586" cvRef="MS" name="contact name" value="Arminja Kettenbach"/> <cvParam accession="MS:1000589" cvRef="MS" name="contact email" value="arminja.n.kettenbach@dartmouth.edu"/> <cvParam accession="MS:1000590" cvRef="MS" name="contact affiliation" value="The Geisel School of Medicine at Dartmouth"/> </Contact> </ContactList> <PublicationList> <Publication id="unpublished"> <cvParam accession="MS:1002853" cvRef="MS" name="Dataset with no associated published manuscript"/> </Publication> </PublicationList> <KeywordList> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="starfish"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="sea star"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="gamete"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="meiosis"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="oocyte"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="prophase"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="PP1"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="PP2A"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="PP2A-B55"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="protein phosphatase"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="phosphorylation"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="dephosphorylation"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="INCENP"/> <cvParam accession="MS:1001925" cvRef="MS" name="submitter keyword" value="TMT"/> </KeywordList> <FullDatasetLinkList> <FullDatasetLink> <cvParam accession="MS:1002488" cvRef="MS" name="MassIVE dataset URI" value="http://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=89f567251a454f64a038dcb613aa2bde"/> </FullDatasetLink> <FullDatasetLink> <cvParam accession="MS:1002852" cvRef="MS" name="Dataset FTP location" value="ftp://massive.ucsd.edu/MSV000085949/"/> </FullDatasetLink> </FullDatasetLinkList> </ProteomeXchangeDataset>