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PXD020912-1

PXD020912 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCitrus sinensis challenged with LotP
DescriptionInfiltrating purified LotP protein in healthy citrus leaf induces chlorosis and provoke plant stress. Further proteomic analysis of the affected tissue using TMT11MS/MS revealed a significant abundance of plant proteins associated with stabilizing and processing mRNA transcripts and miRNAs. Interestingly, a subset of transcription factors directly connected to pathways coordinating the innate plant defense were also more abundant in the damage tissue two days post infiltration. Inhibiting the formation of LotP multimers via direct mutation of critical residues of the N terminal domain significantly decreases the chlorotic response in infiltrated leaf as well as the intensity of the associated proteome reaction.
HostingRepositoryPRIDE
AnnounceDate2021-09-09
AnnouncementXMLSubmission_2021-09-09_00:54:43.669.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD020912
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKaylie Padgett-Pagliai
SpeciesList scientific name: Citrus sinensis; NCBI TaxID: 2711;
ModificationListmethylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-14 04:56:42ID requested
12021-09-09 00:54:44announced
Publication List
Merli ML, Padgett-Pagliai KA, Cuaycal AE, Garcia L, Marano MR, Lorca GL, Gonzalez CF, Defense Response Activation. Front Microbiol, 12():661547(2021) [pubmed]
Keyword List
submitter keyword: Citrus, LC-MS/MS
Contact List
Claudio Fabricio Gonzalez
contact affiliationMicrobiology and Cell Science Department, Genetics Institute, Institute of Food and Agricultural Science, University of Florida, Gainesville, Florida, United States
contact emailcfgonzalez@ufl.edu
lab head
Kaylie Padgett-Pagliai
contact affiliationUniversity of Florida
contact emailkapadgett@ufl.edu
dataset submitter
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Dataset FTP location
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