PXD020902-2

PXD020902 is an original dataset announced via ProteomeXchange.

Title	Fecal metaproteomics reveals reduced gut inflammation and changed microbial metabolism following lifestyle-induced weight loss
Description	Aims Gut microbiota-mediated inflammation promotes obesity-associated low-grade inflammation, which represents a hallmark of the metabolic syndrome (MetS). Lifestyle-induced weight loss (WL) is regarded as an efficient therapy to reverse MetS and to prevent disease progression. The objective of this study was to investigate if lifestyle-induced WL modulates the gut microbiome and its interaction with the host. Methods We analyzed and compared the fecal metaproteome of 33 individuals with MetS in a longitudinal study before and after lifestyle-induced WL in a well-defined cohort (ICTRP Trial Number: U1111-1158-3672). Results The 6-month WL intervention resulted in reduced BMI (-13.9%), increased insulin sensitivity (HOMA-IR; -53.70%) and reduced levels of circulating CRP (-66.86%), indicating MetS reversal. The metaprotein spectra of the host revealed a decrease of human proteins associated with gut inflammation and reduced abundance of human pancreatic alpha-amylase. Surprisingly, taxonomic analysis of the fecal metaproteome revealed only minor changes in the bacterial composition with an increase of low-abundant families (Desulfovibrionaceae, Leptospiraceae, Syntrophomonadaceae, Thermotogaceae, Verrucomicrobiaceae). Yet, we detected increased abundance of microbial metaprotein spectra that correspond to enhanced hydrolysis of complex carbohydrates (endoglucanase A, β-1,4-mannooligosaccharide phosphorylase, galactokinase, 5-keto-D-gluconate 5-reductase), indicating functional changes of the gut microbiome. Conclusions Our results indicate that lifestyle induced WL may improve interaction between the gut microbiome and the host in individuals with MetS, while bacterial composition remained almost stable. Metaproteome analysis of host proteins reveals reduced gut inflammation whereas microbial metaprotein spectra indicate functional changes towards degradation of complex carbohydrates. The filenames correspond to the ID of the patient (1-33), whereas “C” corresponds to the sampling before weight loss and “ABC” to the sampling after weight loss.
HostingRepository	PRIDE
AnnounceDate	2021-04-29
AnnouncementXML	Submission_2021-04-29_10:20:12.788.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Heyer
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-13 22:46:01	ID requested
1	2021-04-19 04:28:08	announced
⏵ 2	2021-04-29 10:20:13	announced	2021-04-29: Updated project metadata.
3	2021-04-29 10:46:02	announced	2021-04-29: Updated project metadata.

Dataset with its publication pending

submitter keyword: metaproteomics, obesity, metabolic syndrome, microbiome, gut inflammation, weight loss

Dirk Benndorf
contact affiliation	Otto von Guericke Universität Magdeburg
contact email	benndorf@mpi-magdeburg.mpg.de
lab head
Robert Heyer
contact affiliation	OvGU
contact email	heyer@mpi-magdeburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020902
     1. Label: PRIDE project
     2. Name: Fecal metaproteomics reveals reduced gut inflammation and changed microbial metabolism following lifestyle-induced weight loss