PXD020890-1

PXD020890 is an original dataset announced via ProteomeXchange.

Title	HDX-MS of TRAPPII reveals a conserved Rab binding interface
Description	We used hydrogen deuterium exchange mass spectrometry (HDX-MS) to define the interface of TRAPPII with three Rab GTPases (Rab1, Rab11, Rab43). These experiments were performed in the presence of EDTA to generate a nucleotide free stabilised Rab-GEF complex. Significant decreases in deuterium incorporation (defined as >4%, >0.4Da, two tailed T-test p value <0.01) were identified in the TRAPPC2L, TRAPPC4, and TRAPPC5 in the presence of Rab GTPases with no other changes in other TRAPP subunits. Here we show that all three Rab GTPases bind at a conserved interface of TRAPPII.
HostingRepository	PRIDE
AnnounceDate	2020-12-11
AnnouncementXML	Submission_2020-12-10_23:22:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	impact HD

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-13 01:30:19	ID requested
⏵ 1	2020-12-10 23:22:27	announced
2	2024-10-22 05:16:33	announced	2024-10-22: Updated project metadata.

Jenkins ML, Harris NJ, Dalwadi U, Fleming KD, Ziemianowicz DS, Rafiei A, Martin EM, Schriemer DC, Yip CK, Burke JE, The substrate specificity of the human TRAPPII complex's Rab-guanine nucleotide exchange factor activity. Commun Biol, 3(1):735(2020) [pubmed]

submitter keyword: HDX-MS, TRAPPII, Rab, GEF

John E Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020890
     1. Label: PRIDE project
     2. Name: HDX-MS of TRAPPII reveals a conserved Rab binding interface