PXD020866-1

PXD020866 is an original dataset announced via ProteomeXchange.

Title	Comparative phosphoproteomics of classical bordetellae elucidates the role of serine, threonine and tyrosine phosphorylation in Bordetella biology and virulence
Description	This study characterised the Ser/Thr/Tyr phosphoproteome of classical Bordetella species and examined its role in Bordetella biology and virulence. This study found 70 unique phosphorylated proteins in the classical bordetellae group with a high degree of conservation observed and phosphorylation was a key regulator of Bordetella metabolism with proteins involved in gluconeogenesis, TCA cycle, amino acid and nucleotide synthesis significantly enriched. We also identified the phosphorylation of three key virulence pathways which separates classical from non-classical bordetellae including the type III secretion system, alcaligin synthesis (the primary siderophore produced by Bordetella) and the BvgAS master transcriptional regulatory system for virulence genes in Bordetella. Seven new phosphosites were identified in BvgA with 6 located in the DNA binding domain. Of the 7 new phosphosites, four were not detected in non-classical bordetellae. This suggests that serine/threonine phosphorylation may play an important role in stabilising or destabilising BvgA binding to DNA for fine-tuning of virulence gene expression and that BvgA phosphorylation may be an important factor that separates classical from non-classical bordetellae. This study provides the first insight into the phosphoproteome of classical Bordetella species and the role Ser/Thr/Tyr phosphorylation plays in Bordetella biology and virulence.
HostingRepository	PRIDE
AnnounceDate	2021-03-26
AnnouncementXML	Submission_2021-03-25_23:18:34.204.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020866
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ruiting Lan
SpeciesList	scientific name: Bordetella pertussis; NCBI TaxID: 520; scientific name: Bordetella parapertussis 12822; NCBI TaxID: 257311; scientific name: Bordetella bronchiseptica RB50; NCBI TaxID: 257310;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-12 01:26:54	ID requested
⏵ 1	2021-03-25 23:18:34	announced

Dataset with its publication pending

submitter keyword: Bordetella pertussis, Bordetella bronchiseptica, Bordetella parapertussis, phosphoproteome, Fusion Lumos, whooping cough, virulence, BvgA

Ruiting Lan
contact affiliation	School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia
contact email	r.lan@unsw.edu.au
lab head
Ruiting Lan
contact affiliation	University of New South Wales
contact email	r.lan@unsw.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020866
     1. Label: PRIDE project
     2. Name: Comparative phosphoproteomics of classical bordetellae elucidates the role of serine, threonine and tyrosine phosphorylation in Bordetella biology and virulence