PXD020862 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PfDOZI protein-protein interaction in plasmodium falciparum |
Description | Here, we characterized an RNA binding protein (RBP), PfDOZI, which is an integral cytoplasmic component of RNP granule. We observed that deleting pfdozi led to an asexual growth advantage due to the escalated merozoite invasion capacity. Also, pfdozi disruption resulted in a significant impairment in gametocyte commitment and morphology development. Through high-throughput RNA-seq, we revealed that PfDOZI affected the transcriptome in both asexual and sexual stages when pfdozi was deleted. Mechanically, we confirmed that PfDOZI could interact with P body like mRNP in the schizont stage, while stress granule-like granule in gametocyte stage. The identification of PfDOZI protein complex in stressed schizont further indicate PfDOZI could shift its function from mRNA degradation to translation repression during stage conversion. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-16 |
AnnouncementXML | Submission_2024-04-15_19:12:15.888.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD020862 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | HUI MIN |
SpeciesList | scientific name: Plasmodium falciparum; NCBI TaxID: 5833; |
ModificationList | No PTMs are included in the dataset |
Instrument | orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-12 01:26:31 | ID requested | |
⏵ 1 | 2024-04-15 19:12:16 | announced | |
2 | 2024-10-22 06:36:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: PfDOZI, asexual stage, sexual stage, Plasmodium falciparum, LC-MS/MS |
Contact List
Liwang Cui |
contact affiliation | Division of Infectious Diseases and International Medicine Department of Internal Medicine Morsani College of Medicine University of South Florida |
contact email | liwangcui@usf.edu |
lab head | |
HUI MIN |
contact affiliation | University of South Florida |
contact email | huim868@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020862
- Label: PRIDE project
- Name: PfDOZI protein-protein interaction in plasmodium falciparum