PXD020862

PXD020862 is an original dataset announced via ProteomeXchange.

Title	PfDOZI protein-protein interaction in plasmodium falciparum
Description	Here, we characterized an RNA binding protein (RBP), PfDOZI, which is an integral cytoplasmic component of RNP granule. We observed that deleting pfdozi led to an asexual growth advantage due to the escalated merozoite invasion capacity. Also, pfdozi disruption resulted in a significant impairment in gametocyte commitment and morphology development. Through high-throughput RNA-seq, we revealed that PfDOZI affected the transcriptome in both asexual and sexual stages when pfdozi was deleted. Mechanically, we confirmed that PfDOZI could interact with P body like mRNP in the schizont stage, while stress granule-like granule in gametocyte stage. The identification of PfDOZI protein complex in stressed schizont further indicate PfDOZI could shift its function from mRNA degradation to translation repression during stage conversion.
HostingRepository	PRIDE
AnnounceDate	2024-04-16
AnnouncementXML	Submission_2024-04-15_19:12:15.888.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020862
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	HUI MIN
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	No PTMs are included in the dataset
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-12 01:26:31	ID requested
⏵ 1	2024-04-15 19:12:16	announced

10.6019/PXD020862;

submitter keyword: PfDOZI, asexual stage, sexual stage, Plasmodium falciparum, LC-MS/MS

Liwang Cui
contact affiliation	Division of Infectious Diseases and International Medicine Department of Internal Medicine Morsani College of Medicine University of South Florida
contact email	liwangcui@usf.edu
lab head
HUI MIN
contact affiliation	University of South Florida
contact email	huim868@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD020862

PRIDE project URI

• PRIDE
  1. PXD020862
     1. Label: PRIDE project
     2. Name: PfDOZI protein-protein interaction in plasmodium falciparum