PXD020859-1

PXD020859 is an original dataset announced via ProteomeXchange.

Title	Cross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes
Description	Synaptic vesicles are storage organelles for neurotransmitters in the pre-synaptic cytosol. When an action potential arrives at the pre-synaptic terminal, they fuse with the pre-synaptic membrane and neurotransmitters are released. In this project, we set out to study the protein interactions formed in synaptic vesicle membranes. For this, we first assessed the proteome of five independent vesicle preparations. Using chemical cross-linking, we then tackled the interactions of the major protein components. To gain further insights, we combined this approach with a chemical labelling strategy. Specific protein interactions were then studied after (i) treating the vesicles with Botulinum neurotoxin B, (ii) binding the soluble SNARE complex, and (iii) fusion of the vesicles with empty liposomes. Our findings reveal stable interaction modules in synaptic vesicles.
HostingRepository	PRIDE
AnnounceDate	2020-12-09
AnnouncementXML	Submission_2020-12-09_00:26:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie Barth
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-11 17:03:58	ID requested
⏵ 1	2020-12-09 00:26:30	announced

Dataset with its publication pending

submitter keyword: Synaptic vesicles, protein networks, chemical cross-linking, mass spectrometry, Synaptobrevin-2, Synaptophysin

Carla Schmidt
contact affiliation	Interdisciplinary Research Centre HALOmem, Charles Tanford Protein Centre, Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle, Germany
contact email	carla.schmidt@biochemtech.uni-halle.de
lab head
Marie Barth
contact affiliation	HALOmem, Martin Luther University Halle-Wittenberg
contact email	marie.barth@bct.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020859

PRIDE project URI

• PRIDE
  1. PXD020859
     1. Label: PRIDE project
     2. Name: Cross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes