PXD020856-1

PXD020856 is an original dataset announced via ProteomeXchange.

Title	Harnessing Ionic Selectivity In Acetyltransferase Chemoproteomic Probes
Description	Chemical proteomics (chemoproteomics) has emerged as a powerful strategy for the high-throughput annotation of protein function. This approach involves using an active site probe to capture an enzyme class of interest from cells, allowing the biological activity of multiple enzymes within that family to be assessed in parallel. Coenzyme A based chemoproteomic probes may have utility in studying the pharmacological interactions of additional enzyme classes, beyond histone acetyltransferases. However, one challenge in applying this approach is how to differentiate a ‘true hit’ from background. Many histone acetyltransferases utilize an ordered binding mechanism, with acetyl-CoA binding first, which allows their chemoproteomic capture to be competed by pre-incubating lysates with acetyl-CoA. However, for acetyltransferases that don’t exhibit this binding mode, or which bind to a different ligand, acetyl-CoA competition may not be evident, limiting our ability to discern selective chemoproteomic enrichment from noise. In this study, two physiochemically distinct capture probes were used to enrich proteins to distinguish true hits from background and identify new targets selectively enriched by CoA-based probes, thus expanding the scope of protein-ligand interactions open to chemoproteomic interrogation.
HostingRepository	PRIDE
AnnounceDate	2021-08-19
AnnouncementXML	Submission_2021-08-18_22:46:57.964.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020856
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michaella Levy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-11 17:00:54	ID requested
⏵ 1	2021-08-18 22:46:58	announced

Dataset with its publication pending

submitter keyword: Acetyltransferase, Affinity Capture, TMT

Laurence Florens
contact affiliation	Stowers Institute for Medical Research
contact email	laf@stowers.org
lab head
Michaella Levy
contact affiliation	Stowers Institute for Medical Research
contact email	mly@stowers.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020856
     1. Label: PRIDE project
     2. Name: Harnessing Ionic Selectivity In Acetyltransferase Chemoproteomic Probes