PXD020845 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometric characterization of HSV-1 L-particles from human dendritic cells and BHK21 cells and analysis of their functional role |
Description | Herpes simplex virus type 1 (HSV-1) is a very common human pathogenic virus among the world’s 12 population. The lytic replication cycle of HSV-1 is, amongst others, characterized by a tripartite viral 13 gene expression cascade, the assembly of nucleocapsids involving their subsequent nuclear egress, 14 tegumentation, re-envelopment and the final release of progeny viral particles. During productive 15 infection of a multitude of different cell types, HSV-1 generates not only infectious heavy (H-) 16 particles, but also non-infectious light (L-) particles, lacking the capsid. In monocyte-derived mature 17 dendritic cells (mDCs), HSV-1 causes a non-productive infection with the predominant release of L-18 particles. Until now, the generation and function of L-particles is not well understood, however, they 19 are described as factors transferring viral components to the cellular microenvironment. To obtain 20 deeper insights into the L-particle composition, we performed a mass-spectrometry-based analysis of 21 L-particles derived from HSV-1-infected mDCs or BHK21 cells and H-particles from the latter one. 22 In total, we detected 63 viral proteins in both H- and L-particle preparations derived from HSV-1-23 infected BHK21 cells. In L-particles from HSV-1-infected mDCs we identified 41 viral proteins 24 which are differentially distributed compared to L-particles from BHK21 cells. In this study, we 25 present data suggesting that L-particles modify mDCs and suppress their T cell stimulatory capacity. 26 Due to the plethora of specific viral proteins incorporated into and transmitted by L-particles, it is 27 tempting to speculate that L-particles manipulate non-infected bystander cells for the benefit of the 28 virus. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_04:45:31.358.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joerg Hofmann |
SpeciesList | scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-11 05:07:40 | ID requested | |
⏵ 1 | 2021-09-09 04:45:32 | announced | |
Publication List
Birzer A, Kraner ME, Heilingloh CS, M, ü, hl-Z, ü, rbes P, Hofmann J, Steinkasserer A, Popella L, Mass Spectrometric Characterization of HSV-1 L-Particles From Human Dendritic Cells and BHK21 Cells and Analysis of Their Functional Role. Front Microbiol, 11():1997(2020) [pubmed] |
Keyword List
submitter keyword: HSV-1, mass spectrometry, heavy particles, light particles, BHK21 cells, dendritic cells, 9 T cell stimulation, immunomodulatory effect |
Contact List
Alexander Steinkasserer |
contact affiliation | Department of Immune Modulation,Universitaetsklinikum Erlangen, Germany |
contact email | steinkasserer@uk-erlangen.de |
lab head | |
Joerg Hofmann |
contact affiliation | FAU Erlangen-Nürnberg |
contact email | joerg.hofmann@fau.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD020845
- Label: PRIDE project
- Name: Mass spectrometric characterization of HSV-1 L-particles from human dendritic cells and BHK21 cells and analysis of their functional role