PXD020820-1

PXD020820 is an original dataset announced via ProteomeXchange.

Title	Full geroprotection from brief rapamycin treatment by persistently increased intestinal autophagy
Description	The licensed drug rapamycin has potential to be repurposed for geroprotection. A key challenge is to avoid the adverse side-effects of clinical dosing regimes. Here we show a profound memory effect of brief rapamycin treatment. Brief, early adult treatment extended lifespan in Drosophila to the same degree as lifelong dosing. Lasting memory of earlier rapamycin treatment was mediated by elevated autophagy in enterocytes of the gut, accompanied by increased intestinal spermidine levels and improved structure and function of the ageing intestine. Brief elevation of autophagy itself induced a long-term increase in autophagy. In mice, short-term, 3-month treatment also induced a full memory effect, with enhanced autophagy in Paneth cells, improved Paneth cell architecture and gut barrier function at levels induced by chronic treatment, even 6 months after rapamycin was withdrawn. Past rapamycin treatment also enhanced the regenerative potential of aged intestine in intestinal organoids. Full geroprotective effects of chronic rapamycin treatment can thus be obtained with a brief pulse of the drug.
HostingRepository	PRIDE
AnnounceDate	2022-07-18
AnnouncementXML	Submission_2022-07-18_05:35:25.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-10 03:46:34	ID requested
⏵ 1	2022-07-18 05:35:25	announced

Dataset with its publication pending

submitter keyword: autophagy, TMT, rapamycin, lifespan, fly, drosophila

Linda Partridge
contact affiliation	Max Planck Institute for Biology of Ageing, Cologne, Germany. Institute of Healthy Ageing, and Department of Genetics, Evolution and Environment, UCL, London, UK.
contact email	Linda.Partridge@age.mpg.de
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD020820

PRIDE project URI

• PRIDE
  1. PXD020820
     1. Label: PRIDE project
     2. Name: Full geroprotection from brief rapamycin treatment by persistently increased intestinal autophagy