PXD020804-1

PXD020804 is an original dataset announced via ProteomeXchange.

Title	The GAR Domain Determines Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin
Description	In this project we aimed to identify the spectrum of protein binders of nucleolin’s glycine arginine reach (GAR) domain. We did so by designing a 15 amino acid peptide derived from the GAR domain sequence, which includes 3 arginine-glycine-glycine (RGG) repeats and we call "R3". We conducted affinity purification with biotinylated R3 peptide, from mouse sciatic nerve axoplasm preparations, followed by mass spectrometry, in order to identify potential GAR interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2021-07-23
AnnouncementXML	Submission_2021-07-22_23:26:42.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-08 15:45:12	ID requested
⏵ 1	2021-07-22 23:26:42	announced

Dataset with its publication pending

submitter keyword: Axonal transport, Nucleolin, Kinesin, Glycine-Arginine rich domain, Plasma membrane localization, RNA binding protein localization

Mike Fainzilber
contact affiliation	Department of Biomolecular Sciences. Weizmann Institute of Science. Rehovot, Israel
contact email	mike.fainzilber@weizmann.ac.il
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD020804

PRIDE project URI

• PRIDE
  1. PXD020804
     1. Label: PRIDE project
     2. Name: The GAR Domain Determines Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin