PXD020759-1

PXD020759 is an original dataset announced via ProteomeXchange.

Title	Quantitative interactomics of Lck-TurboID in living human T cells unveils T cell receptor stimulation-induced proximal Lck interactors
Description	While Lck has been widely recognized to play a pivotal role in the initiation of the T cell receptor (TCR) signaling pathway, an understanding of the precise regulation of Lck in T cells upon TCR activation remains elusive. Investigation of protein-protein interaction (PPI) using proximity labeling techniques such as TurboID has the potential to provide valuable molecular insights into Lck regulatory networks. By expressing Lck-TurboID in Jurkat T cells, we have uncovered a dynamic, short-range Lck protein interaction network upon 30 minutes of TCR stimulation. In this novel application of TurboID, we detected 27 TCR stimulation-induced Lck-proximal interactors in living T cells, including both bona fide and uncanonical Lck interactors, validating the discovery power of this tool. Our results revealed previously unappreciated Lck PPI which may be associated with cytoskeletal rearrangement, ubiquitination of TCR signaling proteins, activation of the MAPK cascade, coalescence of the LAT signalosome, and formation of the immunological synapse. In this study, we demonstrated for the first time in immune cells and for the kinase Lck that TurboID can be utilized to unveil PPI dynamics in living cells at a time scale consistent with TCR signaling.
HostingRepository	PRIDE
AnnounceDate	2020-11-13
AnnouncementXML	Submission_2020-11-13_05:42:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xien Yu Chua
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 23:20:04	ID requested
⏵ 1	2020-11-13 05:42:25	announced

Dataset with its publication pending

submitter keyword: TurboID, Lck, proximity labeling, interactome, T cell receptor

Arthur, Salomon
contact affiliation	Department of molecular biology, cell biology and biochemistry, Laboratory of Molecular Medicine, Brown University, United States
contact email	arthur_salomon@brown.edu
lab head
Xien Yu Chua
contact affiliation	Brown University
contact email	xien_yu_chua@brown.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020759
     1. Label: PRIDE project
     2. Name: Quantitative interactomics of Lck-TurboID in living human T cells unveils T cell receptor stimulation-induced proximal Lck interactors