PXD020757-1

PXD020757 is an original dataset announced via ProteomeXchange.

Title	Mechanisms and Novel Proteins for Iron Oxide Mineral Reduction in the Hyperthermophilic Crenarchaeon Pyrodictium delaneyi
Description	Dissimilatory iron reduction by hyperthermophilic archaea occurs in many geothermal environments and generally relies on microbe-mineral interactions that transform various iron oxide minerals. In this study, the physiology of dissimilatory iron and nitrate reduction was examined in the hyperthermophilic crenarchaeon Pyrodictium delaneyi Su06T. Protein electrophoresis showed that the c-type cytochrome and general protein compositions of P. delaneyi changed when grown on ferrihydrite relative to nitrate. Differential proteomic analyses using tandem mass tagged protein fragments and mass spectrometry detected 660 proteins and differential production of 127 proteins. Among these, two putative membrane-bound molybdopterin-dependent oxidoreductase complexes increased in relative abundance 60- to 3,000-fold and 50-100-fold in cells grown on iron oxide. A putative 8-heme c-type cytochrome was 60-fold more abundant in iron grown cells and was unique to the Pyrodictiaceae. There was also a >14,700-fold increase in a membrane transport protein in iron grown cells. There were no changes in the abundances of flagellin proteins nor a putative nitrate reductase, but a membrane nitric oxide reductase was more abundant on nitrate. These data help to elucidate the mechanisms by which hyperthermophilic crenarchaea generate energy and transfer electrons across the membrane to iron oxide minerals.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-22_16:32:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020757
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Srishti Kashyap
SpeciesList	scientific name: Pyrodictium delaneyi; NCBI TaxID: 1273541;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 23:11:38	ID requested
⏵ 1	2020-12-22 16:32:04	announced

Dataset with its publication pending

submitter keyword: TMT

hyperthermophile

archaea

iron reducer

James F Holden
contact affiliation	Department of Microbiology, University of Massachusetts Amherst, MA, USA
contact email	jholden@microbio.umass.edu
lab head
Srishti Kashyap
contact affiliation	University of Massachusetts Amherst
contact email	skashyap@umass.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020757
     1. Label: PRIDE project
     2. Name: Mechanisms and Novel Proteins for Iron Oxide Mineral Reduction in the Hyperthermophilic Crenarchaeon Pyrodictium delaneyi