PXD020751 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Host-interactor screens of Phytophthora infestans RXLR proteins |
Description | Pathogens modulate host cell structure and function by secreting effectors into host tissues. Effectors generally function by associating with host molecules and co-opting or perturbing their activities. This study aimed at identifying the plant proteins associated with the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans in order to identify the host processes targeted by these effectors. We carried out an in planta screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by co-immunoprecipitation (co-IP) and liquid chromatography tandem mass spectrometry (LC-MS/MS). This generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes that are candidate effector-targeted pathways. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of vesicle trafficking machinery. One member of this family, PexRD31, increased the number of GFP-2xFYVE labeled vesicles in N. benthamiana cells. We also noted an accumulation of FYVE vesicles in areas of N. benthamiana leaves colonized by P. infestans indicating that this pathogen may enhance endosomal trafficking. We hope that the interactome dataset we generated will serve as a useful community resource for functional studies of P. infestans effectors. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-23 |
AnnouncementXML | Submission_2021-03-10_22:05:21.836.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD020751 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jan Sklenar |
SpeciesList | scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Phytophthora infestans; NCBI TaxID: 4787; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-05 10:01:28 | ID requested | |
1 | 2020-09-23 07:06:53 | announced | |
⏵ 2 | 2021-03-10 22:05:22 | announced | 2021-03-11: Updated publication reference for PubMed record(s): 33677602. |
Publication List
Petre B, Contreras MP, Bozkurt TO, Schattat MH, Sklenar J, Schornack S, Abd-El-Haliem A, Castells-Graells R, Lozano-Dur, รก, n R, Dagdas YF, Menke FLH, Jones AME, Vossen JH, Robatzek S, Kamoun S, Win J, Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. Plant Cell, 33(5):1447-1471(2021) [pubmed] |
Keyword List
submitter keyword: Phytophthora infestans, RXLR effector proteins, Nicotiana Benthamiana |
Contact List
Jan Sklenar |
contact affiliation | The Sainsbury Laboratory, Proteomics group, Norwich, UK |
contact email | jan.sklenar@tsl.ac.uk |
lab head | |
Jan Sklenar |
contact affiliation | The Sainsbury Laboratory, Norwich, UK |
contact email | jan.sklenar@tsl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD020751
- Label: PRIDE project
- Name: Host-interactor screens of Phytophthora infestans RXLR proteins