PXD020740-2

PXD020740 is an original dataset announced via ProteomeXchange.

Title	Chemical proteomic profiling reveals protein interactors of the alarmones diadenosine triphosphate and tetraphosphate
Description	The nucleotides diadenosine triphosphate (Ap3A) and diadenosine tetraphosphate (Ap4A) are formed in prokaryotic and eukaryotic cells. Since their concentrations increase significantly upon cellular stress, they are considered to be alarmones triggering stress adaptive processes. However, their cellular roles remain elusive. To elucidate the proteome-wide interactome of Ap3A and Ap4A and thereby gain insights into their cellular roles, we herein report the development of photoaffinity-labeling probes and their employment in chemical proteomics. We demonstrate that the ApnA interactors we identified are involved in many fundamental cellular processes including carboxylic acid and nucleotide metabolism, gene expression, various regulatory processes and cellular response mechanisms and only around half of them are known nucleotide interactors. Our results highlight common functions of these ApnAs across the domains of life, but also identify those that are different for Ap3A or Ap4A. This study provides a rich source for further functional studies of these nucleotides and depicts useful tools for characterization of their regulatory mechanisms in cells.
HostingRepository	PRIDE
AnnounceDate	2022-03-09
AnnouncementXML	Submission_2022-03-09_04:38:26.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lena Krüger
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 09:39:58	ID requested
1	2022-02-16 04:12:30	announced
⏵ 2	2022-03-09 04:38:27	announced	2022-03-09: Updated project metadata.

Dataset with its publication pending

submitter keyword: Photo-affinity labeling, proteomics,ApnA

Andreas Marx
contact affiliation	1Department of Chemistry, University of Konstanz, Konstanz, Germany 2Konstanz Resea1Department of Chemistry, University of Konstanz, Konstanz, Germany 2Konstanz Research School-Chemical Biology, University of Konstanz, Konstanz, Germany
contact email	andreas.marx@uni-konstanz.de
lab head
Lena Krüger
contact affiliation	University of Konstanz
contact email	lena.krueger@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020740
     1. Label: PRIDE project
     2. Name: Chemical proteomic profiling reveals protein interactors of the alarmones diadenosine triphosphate and tetraphosphate