PXD020719-1

PXD020719 is an original dataset announced via ProteomeXchange.

Title	Integration of JAK/STAT receptor-ligand trafficking, signalling and gene expression in Drosophila melanogaster cells
Description	The JAK/STAT pathway is an essential signalling cascade required for multiple processes during both development and for adult homeostasis. A key question in understanding this pathway is how it is regulated in different cell contexts. Here we have examined how endocytic processing contributes to signalling by the single cytokine receptor, Domeless, in Drosophila melanogaster cells. We identify an evolutionarily conserved di-Leu motif that is required for Domeless internalisation and show that endocytosis is required for activation of a subset of Domeless targets. Our data indicate that endocytosis both qualitatively and quantitatively regulates Domeless signalling. STAT92E, the single STAT transcription factor in Drosophila, appears to be the target of endocytic regulation and our studies show that phosphorylation of STAT92E on Tyr704, while necessary, is not always sufficient for target transcription. Finally, we identify a conserved residue, Thr702, which is essential for Tyr704 phosphorylation. Taken together, our findings identify previously unknown aspects of JAK/STAT pathway regulation likely to play key roles in the spatial and temporal regulation of signalling in vivo.
HostingRepository	PRIDE
AnnounceDate	2020-09-29
AnnouncementXML	Submission_2020-09-29_06:25:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adelina E Acosta-Martin
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-03 22:36:57	ID requested
⏵ 1	2020-09-29 06:25:52	announced

Dataset with its publication pending

submitter keyword: endocytosis, JAK/STAT signalling, phosphorylation, Drosophila, LC-MS/MS

Elizabeth Smythe
contact affiliation	Centre for Membrane Interactions and Dynamics, Department of Biomedical Science, University of Sheffield, Sheffield
contact email	e.smythe@sheffield.ac.uk
lab head
Adelina E Acosta-Martin
contact affiliation	University of Sheffield
contact email	a.acosta-martin@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020719
     1. Label: PRIDE project
     2. Name: Integration of JAK/STAT receptor-ligand trafficking, signalling and gene expression in Drosophila melanogaster cells