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PXD020719 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegration of JAK/STAT receptor-ligand trafficking, signalling and gene expression in Drosophila melanogaster cells
DescriptionThe JAK/STAT pathway is an essential signalling cascade required for multiple processes during both development and for adult homeostasis. A key question in understanding this pathway is how it is regulated in different cell contexts. Here we have examined how endocytic processing contributes to signalling by the single cytokine receptor, Domeless, in Drosophila melanogaster cells. We identify an evolutionarily conserved di-Leu motif that is required for Domeless internalisation and show that endocytosis is required for activation of a subset of Domeless targets. Our data indicate that endocytosis both qualitatively and quantitatively regulates Domeless signalling. STAT92E, the single STAT transcription factor in Drosophila, appears to be the target of endocytic regulation and our studies show that phosphorylation of STAT92E on Tyr704, while necessary, is not always sufficient for target transcription. Finally, we identify a conserved residue, Thr702, which is essential for Tyr704 phosphorylation. Taken together, our findings identify previously unknown aspects of JAK/STAT pathway regulation likely to play key roles in the spatial and temporal regulation of signalling in vivo.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdelina E Acosta-Martin
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-03 22:36:57ID requested
12020-09-29 06:25:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: endocytosis, JAK/STAT signalling, phosphorylation, Drosophila, LC-MS/MS
Contact List
Elizabeth Smythe
contact affiliationCentre for Membrane Interactions and Dynamics, Department of Biomedical Science, University of Sheffield, Sheffield
contact emaile.smythe@sheffield.ac.uk
lab head
Adelina E Acosta-Martin
contact affiliationUniversity of Sheffield
contact emaila.acosta-martin@sheffield.ac.uk
dataset submitter
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