PXD020679-1
PXD020679 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic signatures of corals from thermodynamic reefs |
| Description | In this work, I took an iTRAQ approach to profile the proteomes of 12 coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu") and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan") and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the manuscript describing the experiment (Mayfield et al. 2012 J Exp Biol), the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML files (SHVTS-A & SHVTS-B), two MZID files (SHVTS-A & SHVTS-B), and a tab-delineated file that describes which samples were labelled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome that has been uploaded as a fasta file. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific). |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-08-01 |
| AnnouncementXML | Submission_2020-08-01_09:20:38.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Anderson B Mayfield |
| SpeciesList | scientific name: Cladocopium sp. clade C; NCBI TaxID: 293275; scientific name: Seriatopora hystrix; common name: bird's nest coral; NCBI TaxID: 51070; |
| ModificationList | iTRAQ8plex |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-07-31 10:38:12 | ID requested | |
| ⏵ 1 | 2020-08-01 09:20:38 | announced |
Publication List
| Mayfield AB. Proteomic Signatures of Corals from Thermodynamic Reefs. Mayfield AB (2020) Proteomic Signatures of Corals from Thermodynamic Reefs. Microorganisms (in press). |
Keyword List
| submitter keyword: coral reefs, dinoflagellates, proteomics, iTRAQ, symbiosis, thermal biology |
Contact List
| Anderson Mayfield | |
|---|---|
| contact affiliation | University of Miami |
| contact email | abm64@miami.edu |
| lab head | |
| Anderson B Mayfield | |
| contact affiliation | NOAA |
| contact email | anderson.mayfield@noaa.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085863/ |
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