PXD020635-1

PXD020635 is an original dataset announced via ProteomeXchange.

Title	Whole-body proteome of the marine annelid Hediste diversicolor LC-MSMS
Description	Extreme weather events, such as heatwaves, are becoming increasingly frequent, long-lasting, and severe as global climate change continues, shaping marine biodiversity patterns worldwide. Increased risk of overheating and mortality across major taxa have been recurrently observed, jeopardizing the sustainability of ecosystem services. Molecular responses of species, which scale up to physiological and population responses, are determinant processes that modulate species sensitivity or tolerance to extreme weather events. Here, we analysed the whole-body proteome of the intertidal ragworm Hediste diversicolor (Müller, 1776), a keystone species in estuarine ecosystems and an emergent blue bio-resource, to long-lasting heatwaves (24 ºC vs 30 ºC for 1 month). We hypothesized that this species is phenotypically plastic and thus able to acclimate to heatwaves by inducing cytoprotective pathways and modulating energy metabolism to enhance its thermal tolerance and maintain survival. To test these hypotheses, worms were exposed to control (24 ºC) and a +6 ºC increase in water temperature (30 ºC) for a month, after which they were collected for whole-body proteomic (after 28 days of exposure) analysis. Concomitantly, fatty acid analysis (after 28 days of exposure) and cumulative survival, upper thermal tolerance limits and wet weight (after 30 days of exposure) were compared between control and heatwave exposed worms.
HostingRepository	PRIDE
AnnounceDate	2021-05-07
AnnouncementXML	Submission_2021-05-06_22:28:36.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diana Madeira
SpeciesList	scientific name: Hediste diversicolor; NCBI TaxID: 126592;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-29 04:26:46	ID requested
⏵ 1	2021-05-06 22:28:37	announced
2	2024-10-22 05:21:43	announced	2024-10-22: Updated project metadata.

Madeira D, Fernandes JF, Jer, ó, nimo D, Ricardo F, Santos A, Domingues MR, Calado R, Calcium homeostasis and stable fatty acid composition underpin heatwave tolerance of the keystone polychaete Hediste diversicolor. Environ Res, 195():110885(2021) [pubmed]

submitter keyword: Q Exactive, LC-MSMS, PEAKS, polychaete, whole-body

Ricardo Calado
contact affiliation	ECOMARE-Laboratory for Innovation and Sustainability of Marine Biological Resources, CESAM-Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Estrada do Porto de Pesca Costeira, 3830-565 Gafanha da Nazaré, Portugal
contact email	rjcalado@ua.pt
lab head
Diana Madeira
contact affiliation	University of Aveiro
contact email	d.madeira@ua.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020635
     1. Label: PRIDE project
     2. Name: Whole-body proteome of the marine annelid Hediste diversicolor LC-MSMS