PXD020633-1

PXD020633 is an original dataset announced via ProteomeXchange.

Title	Polydisperse molecular architecture of connexin 26/30 heteromeric hemichannels revealed by AFM imaging
Description	Gap junctions are arrays of intercellular channels formed by two hemichannels docked end-to-end. Each hemichannel is a hexamer containing the same or different connexin (Cx) isoform. While homomeric Cxs forms have been largely described functionally and structurally, lesser is known about heteromeric Cx channels. To unveil properties of heteromeric Cx channels is challenging considering the high number of potential subunit arrangements and stoichiometries, even for only combining two Cx isoforms. We engineered an HA tag onto Cx26 or Cx30 subunits and imaged hemichannels that were liganded by Fab-epitope antibody fragments. For Cx26-HA/Cx30 or Cx26/Cx30-HA heteromeric channels, the Fab-HA binding distribution followed a binomial distribution with a maximum of 3 Fab-HA bound. Furthermore, imaged Cx26/Cx30-HA triple liganded by Fab-HA showed multiple arrangements which can be derived from the law of total probabilities. Our results indicate a dominant subunit stoichiometry of 3Cx26:3Cx30 with the most abundant subunit arrangement of Cx26-Cx26-Cx30-Cx26-Cx30-Cx30.
HostingRepository	PRIDE
AnnounceDate	2020-09-04
AnnouncementXML	Submission_2020-09-04_03:12:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie Barth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-29 03:31:21	ID requested
⏵ 1	2020-09-04 03:12:58	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, Connexin

Carla Schmidt
contact affiliation	, IWE ZIK HALOmem, Martin Luther University Halle-Wittenberg, Halle,Germany
contact email	carla.schmidt@biochemtech.uni-halle.de
lab head
Marie Barth
contact affiliation	HALOmem, Martin Luther University Halle-Wittenberg
contact email	marie.barth@bct.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020633
     1. Label: PRIDE project
     2. Name: Polydisperse molecular architecture of connexin 26/30 heteromeric hemichannels revealed by AFM imaging