PXD020613-2

PXD020613 is an original dataset announced via ProteomeXchange.

Title	Novel inhibitor(s) of NO-production for microglia and macrophages
Description	We aimed to identify novel therapeutic compounds (small molecules) to target microglia and macrophage NO production. We set up a screen using a library of 30.000 drug-like compounds in collaboration with the FEM. Using a microglia cell line and primary microglia and macrophages, we identified one compound that inhibits NO production after LPS stimulation. We have used this compound successfully in several in vitro and in vivo experiments, including in an animal model for stroke. The compound was tagged with a biotinylated linker in order to isolate the compound using strepdavidine beads after incubation with cells or with lysate to identify proteins that the compound was bound to.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:59.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Popp
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-28 05:00:29	ID requested
1	2022-01-24 01:53:07	announced
⏵ 2	2024-10-22 05:30:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: compound MS,Mouse, LC-MS, small molecule, Exploris 480, NO-synthesis

Susanne Wolf , Helmut Kettenmann (Charite Ophthalmology/MDC Cellular Neuroscience).
contact affiliation	Charite Ophthalmology / MDC Cellular Neuroscience
contact email	susanne.wolf@charite.de
lab head
Oliver Popp
contact affiliation	MDC Berlin
contact email	oliver.popp@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD020613

PRIDE project URI

• PRIDE
  1. PXD020613
     1. Label: PRIDE project
     2. Name: Novel inhibitor(s) of NO-production for microglia and macrophages